Immunofluorescent analysis of FCP1 showing staining in the nucleus of HCT 116 cells. HCT 116 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a FCP1 polyclonal antibody (Product # PA5-30302) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 81 and 309 of Human FCP1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30302 targets FCP1 in WB applications and shows reactivity with Human samples.
The PA5-30302 immunogen is recombinant fragment corresponding to a region within amino acids 81 and 309 of Human FCP1.
This gene encodes a protein which interacts with the carboxy-terminus of transcription initiation factor TFIIF, a transcription factor which regulates elongation as well as initiation by RNA polymerase II. The protein may also represent a component of an RNA polymerase II holoenzyme complex. Alternative splicing of this gene results in two transcript variants encoding 2 different isoforms.
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