|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human GFAP fusion protein|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9565 detects GFAP in human, mouse and rat samples.
PA1-9565 has been successfully used in Western blot, immunohistochemistry, and immunocytochemistry procedures.
The PA1-9565 immunogen is a human GFAP fusion protein.
Glial fibrillary acidic protein (GFAP) is a member of the class III intermediate filament protein family. It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells frequently strongly express GFAP. Antibodies to GFAP are therefore very useful as markers of astrocytic cells. In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Amacrine cells coupled to ganglion cells via gap junctions are highly vulnerable in glaucomatous mouse retinas.
PA1-9565 was used in immunohistochemistry - frozen section to examine the structural and functional integrity of amacrine cells in glaucoma samples
|Akopian A,Kumar S,Ramakrishnan H,Viswanathan S,Bloomfield SA||The Journal of comparative neurology (null:null)||2016|
Age-related changes in the optic nerve of Sprague-Dawley rats: an ultrastructural and immunohistochemical study.
PA1-9565 was used in immunohistochemistry - paraffin section to study age-related changes to the optic nerve using rats
|Yassa HD||Acta histochemica (116:1085)||2014|
Fasudil protects hippocampal neurons against hypoxia-reoxygenation injury by suppressing microglial inflammatory responses in mice.
PA1-9565 was used in immunohistochemistry to study the mechanism by which a Rho kinase inhibitor protects hippocampal neurons against hypoxia-reoxygenation injury
|Ding J,Li QY,Wang X,Sun CH,Lu CZ,Xiao BG||Journal of neurochemistry (114:1619)||2010|