Invitrogen

GFAP Monoclonal Antibody (GA5), eFluor™ 660, eBioscience™

Product Image Gallery

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 10 µg/mL of Mouse IgG1 K Isotype Control eFluor® 660 (left) or 10 µg/mL of Anti-Glial Fibrillary Acidic Protein (GFAP) eFluor® 660 (right). Nuclei are stained with DAPI.

GFAP Antibody in Immunocytochemistry, Immunohistochemistry (Frozen) (ICC/IF, IHC (F)) — Figure 3 Serpina3n Expression Is Restricted to Neurons During EAE Representative images of Serpina3n expression in neurons (A-B) and astrocytes (C-D) of PBS (controls) or EAE mice at d16 and d50 postimmunization. Tissues were stained for Serpina3n (red), beta-III-Tubulin (neuronal marker, green), and DAPI (blue) (A-B). Colocalization of Serpina3n in astrocytes was evaluated by staining for Serpina3n (red), GFAP (astrocyte marker, green), and DAPI (blue) (C-D). EAE = experimental autoimmune encephalomyelitis; GFAP = glial fibrillary acid protein.

GFAP Antibody in Immunocytochemistry (ICC/IF) — Figure 3 Data (median and interquartile ranges) for ( a ) the area covered by GFAP fluorescent immunoreactivity and the numerical density of ( c ) S100beta cells and ( e ) NG2-glia immunoreactive puncta (n = 6 in all groups) in the right striatum adjacent to the microinjection site in the germinal matrix of newborn male rats. Symbols represent values obtained for each rat. * p < 0.05 and ** p < 0.01 in each comparison indicated. Note the control values and the effects of collagenase and collagenase plus ACTH. ( b , d , and f ) are representative images of the corresponding results in each experimental group and biomarker studied. Scale bar = 25 um.

GFAP Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Figure 4 S1004A Expression in CNS Tissue During EAE Representative images are shown for S100A4 expression in neurons (A-B) and astrocytes (C-D) of PBS (controls) or EAE mice at d16 and d50 postimmunization. Tissues were stained for S100A4 (red), beta-III-Tubulin (neuronal marker, green), and DAPI (blue) (A-B). Colocalization of S100A4 in astrocytes was evaluated by staining for S100A4 (red), GFAP (astrocyte marker, green), and DAPI (blue) (C-D). EAE = experimental autoimmune encephalomyelitis; GFAP = glial fibrillary acid protein.

GFAP Antibody in Immunohistochemistry (IHC) — Fig 1 Characterization of brain astrocytes. Astrocytes from B6 mice were isolated as described in Materials and Methods. Before use, astrocytes were stained with mAb against GFAP (green) and nuclei with DAPI (blue) followed by immunofluorescence microscopy (A, 20 magnifications) and flow cytometry (B).

GFAP Antibody in Western Blot (WB) — Figure 1 Characterization of neuronal (NDE) and astrocyte derived exosomes (ADE)s. (A) FACS analysis of plasma NDEs and ADEs following the formation of bead-antibody-exosome (BAE)--FITC complexes. Streptavidin magnetic beads were incubated with exosomes isolated from non-psychiatric comparison subjects (NC) and people with schizophrenia (PWS) ( n = 60) and enriched against biotinylated anti-human CD171 biotin (L1CAM, NDE) or anti-GLAST antibody (ADE). BAE complexes are stained with FITC prior to FACS. (B) Plasma NDE and ADE preparations from NC and PWS patients were probed with exosome marker, Flotilin-1 (1:1000). Non-exosome fraction (supernant resulting from 1 h spin at 1500G) served as the negative control while total exosomes (diluted in 1x PBS prior to neuronal and astrocyte enrichment) served as the positive control. The resultant Western Blot demonstrated that NDEs are Flotillin and Neun positive and GFAP negative. However, we were not able to get a signal for the ADEs. Due to multiple freeze-thaw cycles, the integrity of the samples used in the current study had diminished significantly.

GFAP Antibody in Flow Cytometry (Flow) — Figure 5. Depletion of microglia enhanced astrocyte response after brain ischemia. C57BL/6 mice were fed with PLX3397 or control diet for 21 days prior to MCAO. At 24 h after ischemia and reperfusion, single cell suspensions were prepared from brain or spleen tissues of MCAO mice receiving PLX3397 or control diet. (a) Representative flow cytometry plots and bar graph show astrocytes (GFAP + ) in MCAO mice receiving PLX3397 or control diet, at 24 h after ischemia and reperfusion. (b)-(c) Representative plots (b) and bar graphs (c) of flow cytometry analysis show the expression of IL-1alpha, IL-1beta, iNOS, CCL2, TNF-alpha, IL-6 in astrocytes obtained at 24 h after MCAO from mice receiving PLX3397 or control diet. n = 15 mice per group. Error bars represent SD; * P < 0.05; ** P < 0.01.

Product Details

50-9892-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 8 publications 8 publications
Immunohistochemistry (Paraffin) (IHC (P))	10 µg/mL	-
Immunohistochemistry (Frozen) (IHC (F))	10 µg/mL	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	Assay-Dependent	View 4 publications 4 publications
Flow Cytometry (Flow)	Assay-Dependent	View 4 publications 4 publications

Product Specifications
Species Reactivity	Chicken, Human, Mouse, Pig, Rabbit, Rat
Published species	Human, Mouse
Host/Isotype	Mouse / IgG1
Recommended Isotype Control	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 660, eBioscience™
Class	Monoclonal
Type	Antibody
Clone	GA5
Conjugate	eFluor™ 660 Alexa Fluor 488 eFluor 570 eFluor 615 Unconjugated
Excitation/Emission Profile	View spectra
Form	Liquid
Concentration	0.2 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% gelatin
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2574408

Product Specific Information

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.

Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This GA5 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily and specifically expressed in astrocytes and certain astroglia of the central nervous system, in satellite cells of peripheral ganglia, and in non-myelinating Schwann cells of peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Studies of involvement of G-protein coupled receptor-3 in cannabidiol effects on inflammatory responses of mouse primary astrocytes and microglia.

PloS one

Wu J,Chen N,Liu Y,Godlewski G,Kaplan HJ,Shrader SH,Song ZH,Shao H

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Thu Oct 21 00:00:00 EDT 2021

Developmental Role of Adenosine Kinase in the Cerebellum.

eNeuro

Gebril H,Wahba A,Zhou X,Lai T,Alharfoush E,DiCicco-Bloom E,Boison D

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Fri Jul 02 00:00:00 EDT 2021

Regionally diverse astrocyte subtypes and their heterogeneous response to EAE.

Glia

Borggrewe M,Grit C,Vainchtein ID,Brouwer N,Wesseling EM,Laman JD,Eggen BJL,Kooistra SM,Boddeke EWGM

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Plasma Levels of Neuron- and Astrocyte-Derived Exosomal Amyloid Beta1-42, Amyloid Beta1-40, and Phosphorylated Tau Levels in Schizophrenia Patients and Non-psychiatric Comparison Subjects: Relationships With Cognitive Functioning and Psychopathology.

Frontiers in psychiatry

Lee EE,Winston-Gray C,Barlow JW,Rissman RA,Jeste DV

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Western Blot

Sat Mar 27 00:00:00 EDT 2021

CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.

Neurology(R) neuroimmunology & neuroinflammation

Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

CSF SERPINA3 Levels Are Elevated in Patients With Progressive MS.

Neurology(R) neuroimmunology & neuroinflammation

Fissolo N,Matute-Blanch C,Osman M,Costa C,Pinteac R,Miró B,Sanchez A,Brito V,Dujmovic I,Voortman M,Khalil M,Borràs E,Sabidó E,Issazadeh-Navikas S,Montalban X,Comabella Lopez M

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Mon Mar 01 00:00:00 EST 2021

Muscovite nanoparticles mitigate neuropathic pain by modulating the inflammatory response and neuroglial activation in the spinal cord.

Neural regeneration research

Oh JY,Hwang TY,Jang JH,Park JY,Ryu Y,Lee H,Park HJ

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry (Frozen)

Sun Nov 01 00:00:00 EDT 2020

Neuroprotective effect of ACTH on collagenase-induced peri-intraventricular hemorrhage in newborn male rats.

Scientific reports

Martins CA,Neves LT,de Oliveira MMBP,Bagatini PB,Barboza R,Mestriner RG,Xavier LL,Rasia-Filho AA

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunocytochemistry

Tue Oct 20 00:00:00 EDT 2020

Roles of Pannexin-1 Channels in Inflammatory Response through the TLRs/NF-Kappa B Signaling Pathway Following Experimental Subarachnoid Hemorrhage in Rats.

Frontiers in molecular neuroscience

Wu LY,Ye ZN,Zhou CH,Wang CX,Xie GB,Zhang XS,Gao YY,Zhang ZH,Zhou ML,Zhuang Z,Liu JP,Hang CH,Shi JX

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunofluorescence

Thu Oct 01 00:00:00 EDT 2020

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.

Frontiers in pharmacology

Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Inhibition of GSK3β and RIP1K Attenuates Glial Scar Formation Induced by Ischemic Stroke via Reduction of Inflammatory Cytokine Production.

Frontiers in pharmacology

Liu J,Zhu YM,Guo Y,Lin L,Wang ZX,Gu F,Dong XY,Zhou M,Wang YF,Zhang HL

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunocytochemistry

Mon Sep 28 00:00:00 EDT 2020

Bidens pilosa Extract Administered after Symptom Onset Attenuates Glial Activation, Improves Motor Performance, and Prolongs Survival in a Mouse Model of Amyotrophic Lateral Sclerosis.

Oxidative medicine and cellular longevity

Kosuge Y,Kaneko E,Nango H,Miyagishi H,Ishige K,Ito Y

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunocytochemistry

Thu Sep 17 00:00:00 EDT 2020

Flow-cytometric microglial sorting coupled with quantitative proteomics identifies moesin as a highly-abundant microglial protein with relevance to Alzheimer's disease.

Molecular neurodegeneration

Rayaprolu S,Gao T,Xiao H,Ramesha S,Weinstock LD,Shah J,Duong DM,Dammer EB,Webster JA,Lah JJ,Wood LB,Betarbet R,Levey AI,Seyfried NT,Rangaraju S

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunohistochemistry

Thu May 07 00:00:00 EDT 2020

Pseudovirus rVSVΔG-ZEBOV-GP Infects Neurons in Retina and CNS, Causing Apoptosis and Neurodegeneration in Neonatal Mice.

Cell reports

McWilliams IL,Kielczewski JL,Ireland DDC,Sykes JS,Lewkowicz AP,Konduru K,Xu BC,Chan CC,Caspi RR,Manangeeswaran M,Verthelyi D

50-9892 was used in Immunohistochemistry to show that a single inoculation of rVSVΔG-ZEBOV-GP virus in neonatal C57BL/6 mice results in transient viremia, neurological symptoms, high viral titers in eyes and brains, and death.

Tue Feb 12 00:00:00 EST 2019

FGL2 promotes tumor progression in the CNS by suppressing CD103⁺ dendritic cell differentiation.

Nature communications

Yan J,Zhao Q,Gabrusiewicz K,Kong LY,Xia X,Wang J,Ott M,Xu J,Davis RE,Huo L,Rao G,Sun SC,Watowich SS,Heimberger AB,Li S

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Immunofluorescence

Fri Jan 25 00:00:00 EST 2019

Nicotine promotes neuron survival and partially protects from Parkinson's disease by suppressing SIRT6.

Acta neuropathologica communications

Nicholatos JW,Francisco AB,Bender CA,Yeh T,Lugay FJ,Salazar JE,Glorioso C,Libert S

50-9892 was used in Western Blotting to determine the role of nicotine on the abundance of SIRT6 in Parkinson's.

Thu Nov 08 00:00:00 EST 2018

Role of miR-146a in neural stem cell differentiation and neural lineage determination: relevance for neurodevelopmental disorders.

Molecular autism

Nguyen LS,Fregeac J,Bole-Feysot C,Cagnard N,Iyer A,Anink J,Aronica E,Alibeu O,Nitschke P,Colleaux L

50-9892 was used in Flow cytometry/Cell sorting to assess the role of miR-146a in early brain development.

Wed Oct 24 00:00:00 EDT 2018

Depletion of microglia exacerbates postischemic inflammation and brain injury.

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism

Jin WN,Shi SX,Li Z,Li M,Wood K,Gonzales RJ,Liu Q

Thu Jun 01 00:00:00 EDT 2017

Atoxic derivative of botulinum neurotoxin A as a prototype molecular vehicle for targeted delivery to the neuronal cytoplasm.

PloS one

Vazquez-Cintron EJ,Vakulenko M,Band PA,Stanker LH,Johnson EA,Ichtchenko K

Published figure using GFAP monoclonal antibody (Product # 50-9892-82) in Flow Cytometry

Wed Dec 03 00:00:00 EST 2014

CD36-mediated hematoma absorption following intracerebral hemorrhage: negative regulation by TLR4 signaling.

Journal of immunology (Baltimore, Md. : 1950)

Fang H,Chen J,Lin S,Wang P,Wang Y,Xiong X,Yang Q

50-9892 was used in Flow cytometry/Cell sorting to study the function and potential mechanism of CD36 in hematoma absorption using in vitro and in vivo intracerebral hemorrhage models.

Sun Jun 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Astrocyte or Intermediate Filament Protein; FLJ45472; GFAP; GFAP epsilon; Glial fibrillary acidic protein; Glial Fibrillary Acidic Protein (GFAP); glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

Gene Aliases: AI836096; ALXDRD; GFAP

UniProt ID: (Human) Q9UFD0, (Rat) P47819, (Mouse) P03995

Entrez Gene ID: (Rabbit) 100352532, (Pig) 396562, (Chicken) 419969, (Human) 2670, (Rat) 24387, (Mouse) 14580

Function(s)