Invitrogen

GFAP Monoclonal Antibody (GA5), eFluor 660, eBioscience™

Product Image Gallery

GFAP Antibody — FIGURE 4 The expression and distribution of Pannexin-1 Channels were detected by double immunofluorescent staining. Pannexin-1 Channels/NeuN, Pannexin-1 Channels/IBA and Pannexin-1 Channels/GFAP co-staining in the Sham group and SAH group (Pannexin-1 Channel = red, NeuN, IBA and GFAP = green, DAPI = blue). According to the result of immunofluorescence staining, at the cellular level, Pannexin-1 Channels were expressed mainly in neurons and microglial cells, whereas infrequently expressed in astrocytes. Data not shown.

GFAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of formalin-fixed paraffin embedded human cerebellum using 10 µg/mL of Mouse IgG1 K Isotype Control eFluor® 660 (left) or 10 µg/mL of Anti-Glial Fibrillary Acidic Protein (GFAP) eFluor® 660 (right). Nuclei are stained with DAPI.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

Assay-Dependent

Immunocytochemistry (ICC)

Assay-Dependent

Immunofluorescence (IF)

Assay-Dependent

Immunohistochemistry (Frozen) (IHC (F))

10 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

10 µg/mL

Published Applications

Immunofluorescence (IF)

See 1 publication below

Flow Cytometry (Flow)

See 3 publications below

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Pig, Rabbit, Rat

Published species

Mouse

Host / Isotype

Mouse / IgG1

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor 660, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GA5

Conjugate

eFluor® 660

Excitation/Emission Profile

View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.1% gelatin

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2574408

Product Specific Information

Description: This GA5 monoclonal antibody reacts with human, mouse, rat, chicken, rabbit, and pig glial fibrillary acidic protein (GFAP). This 49-kDa type III intermediate filament protein is expressed in neural tissues and distinguishes astrocytes from other glial cells during central nervous system development. Three alternative splice variants of GFAP exist; however, alpha-GFAP is the predominant form expressed in astrocytes. GFAP can co-assemble with vimentin and nestin in astrocytes, but such associations are not required for assembly. Like other intermediate filaments, GFAP assembly is dependent on phosphorylation and dephosphorylation of the N-terminal domain. Studies have demonstrated that mutations in the GFAP gene lead to Alexander disease. Moreover, GFAP has also been shown to be overexpressed in certain glial-derived tumors.

Applications Reported: This GA5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining of frozen and formalin-fixed paraffin embedded tissue sections, and immunocytochemistry.

Applications Tested: This GA5 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

GFAP (Glial fibrillary acidic protein) is a member of the class III intermediate filament protein family. GFAP is heavily, and specifically, expressed in astrocytes and certain astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In addition, neural stem cells strongly express GFAP. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes. GFAP is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing of the GFAP gene results in multiple transcript variants encoding distinct isoforms.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: FLJ45472; GFAP; Glial fibrillary acidic protein; glial fibrillary acidic protein alpha; intermediate filament; intermediate filament protein

Gene Aliases: AI836096; ALXDRD; GFAP

UniProt ID: (Human) P14136, (Rat) P47819, (Mouse) P03995

Entrez Gene ID: (Rabbit) 100352532, (Pig) 396562, (Human) 2670, (Rat) 24387, (Mouse) 14580

Molecular Function: cytoskeletal protein intermediate filament structural protein