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GLUT1 Polyclonal Antibody

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GLUT1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HeLa cells using 21829-1-AP (GLUT1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GLUT1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 21829-1-AP (SLC2A1,GLUT1 Antibody) at dilution of 1:200 (under 40x lens).

GLUT1 Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 21829-1-AP (GLUT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

GLUT1 Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 0.2ug SLC2A1,GLUT1 antibody (21829-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

21829-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 35 publications 35 publications
Immunohistochemistry (IHC)	Assay-dependent	View 8 publications 8 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	View 4 publications 4 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Mouse, Rat, Human
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GLUT1 Fusion Protein Ag16282 (216-280 aa encoded by BC121804) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.23 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

21829-1-AP antibody can also detect the 25 kDa degradation protein in SDS-PAGE.

Immunogen sequence: INRNEENRA KSVLKKLRGT ADVTHDLQEM KEESRQMMRE KKVTILELFR SPAYRQPILI AVVLQL (216-280 aa encoded by BC121804)

Target Information

Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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miR-328 mediates a metabolic shift in colon cancer cells by targeting SLC2A1/GLUT1.

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Bioinformatics

Protein Aliases: choreoathetosis/spasticity, episodic (paroxysmal choreoathetosis/spasticity); glucose transporter glut1; Glucose transporter type 1, erythrocyte/brain; GLUT-1; GT1; HepG2 glucose transporter; human T-cell leukemia virus (I and II) receptor; MGC141895; MGC141896; receptor for HTLV-1 and HTLV-2; solute carrier family 2 (facilitated glucose transporter), member 1; Solute carrier family 2 a 1 (facilitated glucose transporter) brain; Solute carrier family 2, facilitated glucose transporter member 1; solute carrier family 2, member 1

Gene Aliases: CSE; DYT17; DYT18; DYT9; EIG12; GLUT; GLUT-1; GLUT1; GLUT1DS; GLUTB; GTG1; Gtg3; HTLVR; PED; RATGTG1; SDCHCN; SLC2A1

UniProt ID: (Human) P11166, (Mouse) P17809, (Rat) P11167

Entrez Gene ID: (Human) 6513, (Mouse) 20525, (Rat) 24778

Function(s)

protein complex assembly transport carbohydrate transport glucose transport proton transport hexose transmembrane transport cellular response to glucose starvation xenobiotic transport glucose import transmembrane transport dehydroascorbic acid transport cation transmembrane transport lactose biosynthetic process response to osmotic stress L-ascorbic acid metabolic process regulation of insulin secretion glucose transmembrane transport

Process(es)

glucose transmembrane transporter activity protein binding kinase binding dehydroascorbic acid transporter activity identical protein binding xenobiotic transporter activity protein self-association D-glucose transmembrane transporter activity transporter activity sugar:proton symporter activity transmembrane transporter activity substrate-specific transmembrane transporter activity

GLUT1 Polyclonal Antibody

Product Details

21829-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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