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GOLPH3 Polyclonal Antibody

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GOLPH3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

GOLPH3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 19112-1-AP (GOLPH3 antibody) at dilution of 1:50 (under 40x lens).

GOLPH3 Antibody in Western Blot (WB) — Mouse lung tissue were subjected to SDS PAGE followed by western blot with 19112-1-AP (GOLPH3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

GOLPH3 Antibody in Immunoprecipitation (IP) — IP result of anti-GOLPH3 (IP:19112-1-AP, 3ug; Detection:19112-1-AP 1:1500) with mouse testis tissue lysate 4000ug.

Product Details

19112-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 5 publications 5 publications
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	GOLPH3 Fusion Protein Ag5443 (1-298 aa encoded by BC033725) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MTSLTQRSS GLVQRRTEAS RNAADKERAA GGGAGSSEDD AQSRRDEQDD DDKGDSKETR LTLMEEVLLL GLKDREGYTS FWNDCISSGL RGCMLIELAL RGRLQLEACG MRRKSLLTRK VICKSDAPTG DVLLDEALKH VKETQPPETV QNWIELLSGE TWNPLKLHYQ LRNVRERLAK NLVEKGVLTT EKQNFLLFDM TTHPLTNNNI KQRLIKKVQE AVLDKWVNDP HRMDRRLLAL IYLAHASDVL ENAFAPLLDE QYDLATKRVR QLLDLDPEVE CLKANTNEVL WAVVAAFTK (1-298 aa encoded by BC033725 )

Target Information

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signaling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signaling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 EDT 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 EDT 2020

CENPH Inhibits Rapamycin Sensitivity by Regulating GOLPH3-dependent mTOR Signaling Pathway in Colorectal Cancer.

Journal of Cancer

Wu W,Wu F,Wang Z,Di J,Yang J,Gao P,Jiang B,Su X

Wed Sep 30 00:00:00 EDT 2020

STK25-induced inhibition of aerobic glycolysis via GOLPH3-mTOR pathway suppresses cell proliferation in colorectal cancer.

Journal of experimental & clinical cancer research : CR

Wu F,Gao P,Wu W,Wang Z,Yang J,Di J,Jiang B,Su X

Wed Jul 11 00:00:00 EDT 2018

miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.

Theranostics

Zhang Q,Zhuang J,Deng Y,Yang L,Cao W,Chen W,Lin T,Lv X,Yu H,Xue Y,Guo H

Mon Jul 09 00:00:00 EDT 2018

miR34a/GOLPH3 Axis abrogates Urothelial Bladder Cancer Chemoresistance via Reduced Cancer Stemness.

Theranostics

Zhang Q,Zhuang J,Deng Y,Yang L,Cao W,Chen W,Lin T,Lv X,Yu H,Xue Y,Guo H

Mon Jul 09 00:00:00 EDT 2018

Re-evaluating the roles of myosin 18Aα and F-actin in determining Golgi morphology.

Cytoskeleton (Hoboken, N.J.)

Bruun K,Beach JR,Heissler SM,Remmert K,Sellers JR,Hammer JA

Mon May 01 00:00:00 EDT 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 EDT 2017

GOLPH3 induces epithelial-mesenchymal transition via Wnt/β-catenin signaling pathway in epithelial ovarian cancer.

Cancer medicine

Sun J,Yang X,Zhang R,Liu S,Gan X,Xi X,Zhang Z,Feng Y,Sun Y

Sat Apr 01 00:00:00 EDT 2017

GOLPH3 high expression predicts poor prognosis in patients with resected non-small cell lung cancer: an immunohistochemical analysis.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Zhang Y,Ma M,Han B

Sat Nov 01 00:00:00 EDT 2014

GOLPH3 is a novel marker of poor prognosis and a potential therapeutic target in human renal cell carcinoma.

British journal of cancer

Xue Y,Wu G,Liao Y,Xiao G,Ma X,Zou X,Zhang G,Xiao R,Wang X,Liu Q,Long D,Yang J,Xu H,Liu F,Liu M,Xie K,Huang R

Tue Apr 29 00:00:00 EDT 2014

GOLPH3 predicts survival of colorectal cancer patients treated with 5-fluorouracil-based adjuvant chemotherapy.

Journal of translational medicine

Wang Z,Jiang B,Chen L,Di J,Cui M,Liu M,Ma Y,Yang H,Xing J,Zhang C,Yao Z,Zhang N,Dong B,Ji J,Su X

Tue Jan 21 00:00:00 EST 2014

GOLPH3 predicts survival of colorectal cancer patients treated with 5-fluorouracil-based adjuvant chemotherapy.

Journal of translational medicine

Wang Z,Jiang B,Chen L,Di J,Cui M,Liu M,Ma Y,Yang H,Xing J,Zhang C,Yao Z,Zhang N,Dong B,Ji J,Su X

Tue Jan 21 00:00:00 EST 2014

Overexpression of GOLPH3 is associated with poor clinical outcome in gastric cancer.

Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine

Hu BS,Hu H,Zhu CY,Gu YL,Li JP

Fri Feb 01 00:00:00 EST 2013

Increased expression of Golgi phosphoprotein-3 is associated with tumor aggressiveness and poor prognosis of prostate cancer.

Diagnostic pathology

Hua X,Yu L,Pan W,Huang X,Liao Z,Xian Q,Fang L,Shen H

Mon Sep 24 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Coat protein GPP34; coat-protein; golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3; golgi phosphoprotein 3 (coat-protein); golgi protein; golgi-associated protein; MIDAS; Mitochondrial DNA absence factor; trans-Golgi protein GMx33

Gene Aliases: 4733401N08Rik; 5730410D03Rik; AW413496; Gmx33; GOLPH3; GOPP1; GPP34; MIDAS; Vps74

UniProt ID: (Human) Q9H4A6, (Mouse) Q9CRA5

Entrez Gene ID: (Human) 64083, (Mouse) 66629, (Rat) 78961

Function(s)

transport Golgi organization cell proliferation glycoprotein biosynthetic process protein secretion gene expression regulation of mitochondrion organization protein transport cell migration lamellipodium assembly positive regulation of TOR signaling Golgi to plasma membrane protein transport negative regulation of apoptotic process protein retention in Golgi apparatus Golgi vesicle budding positive regulation of protein secretion leukocyte tethering or rolling cell adhesion molecule production Golgi ribbon formation asymmetric Golgi ribbon formation

Process(es)

protein binding enzyme binding phosphatidylinositol-4-phosphate binding lipid binding

GOLPH3 Polyclonal Antibody

Product Details

19112-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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