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Invitrogen
GPR32 Polyclonal Antibody
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FIGURE: 1 / 2
GPR32 Antibody (PA3-021) in IHC (P)
Immunohistochemistry analysis of GPR32 was performed on neuroendocrine cells in human small intestine tissue. To expose target proteins, antigen retrieval was performed by microwaving tissues for 20 minutes in 10mM sodium citrate buffer (pH 6.0). Tissue slides were probed with a GPR32 polyclonal antibody (Product # PA3-021) at a dilution of 1:3000, overnight at 4C in a humidified chamber. Tissues were washe... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA3-021
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Cat,
Hamster,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated Cys-LARAFGEEEFLSS-Abu-PRGNAPRE
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
IHC (P) analysis shows positive staining of GPR32 in human small intestine tissue neuroendocrine cells.
WB analysis shows GPR32 in glycoprotein-enriched fractions from GPR32 overexpressing 293 cells. Additional unknown bands at ~30kD and ~130kD were also detected.
While GPR32 has a theoretical MW of ~ 40kD, the observed band in a WB runs at ~60kD due to enrichment of the glycosylated receptor.
Target Information
The G-protein-coupled receptor (GPCR) superfamily is comprised of an estimated 600-1,000 members and is the largest known class of molecular targets with proven therapeutic value. The intronless GPR32 gene encodes an orphan G protein-coupled receptor that binds to resolvin D1 and lipoxin A4. Resolvin D1 binding to GPR32 has been linked to resolution of pulmonary inflammation, suppression of TGFbeta induced epithelial mesenchymal transition of A549 lung cancer cells. Resolvins are being evaluated as new drug candidates acting at the resolution phase of chronic inflammation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GPCRW; resolvin D1 receptor; RVDR
Gene Aliases: DRV1; RVDR1
UniProt ID: (Human) O75388
Entrez Gene ID: (Human) 2854
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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