Immunofluorescent analysis of GRASP65 (green) showing staining in the Golgi apparatus of HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a GRASP65 polyclonal antibody (Product # PA3-910) in 3% BSA-PBS at a dilution of 1:200 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 100x.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||GST fusion protein containing C-terminal residues (202-447) of rat Grasp65.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-910 detects Grasp65 from human, mouse, and rat samples.
PA3-910 has been successfully used in Western blot, IHC (paraffin), immunofluorescence, immunocytochemistry and immunoprecipitation procedures.
The PA3-910 immunogen is a GST fustion protein containing the C-terminal residues (202-447) of rat Grasp65.
Grasp65 is a structural protein of the golgi apparatus. It has been implicated in the stacking of golgi cisternae. Grasp65 is shown to have the properties to bind surfaces together in a mitotically regulated matter.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.