Invitrogen

GRPR Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (5)
Published Figures (2)

GRPR Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 7

GRPR Antibody (OPA1-15619) in IHC (P)

Immunohistochemical (Paraffin) analysis of human skin, melanoma tissue using (Product # OPA1-15619) GRPR Polyclonal Antibody. Antigen retrieval: Heat-induced antigen retrieval.

Product Image Gallery

GRPR Antibody in Immunohistochemistry (IHC) — Antibody staining in gastrin-releasing peptide receptor (GRPR)-positive melanoma tissues. (a,b) GRPR immunoexpression in melanoma, showing strong positive staining. Original magnification (a) x100; (b) x400.

Product Details

OPA1-15619

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 6 publications 6 publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide, conjugated to KLH, corresponding to the 2nd extracellular loop of human GRPR.

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2114349

Product Specific Information

Recommended positive controls: Human pancreas, islets of Langerhans.

By immunohistochemistry, OPA1-15619 detects GRPR in formalin fixed, paraffin embedded human tissues.

Target Information

Gastrin-releasing peptide (GRP) regulates numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation and is a potent mitogen for neoplastic tissues. The effects of GRP are mediated through the gastrin-releasing peptide receptor. This receptor is a glycosylated, 7-transmembrane G-protein coupled receptor that activates the phospholipase C signaling pathway. The receptor is aberrantly expressed in numerous cancers such as those of the lung, colon, and prostate. An individual with autism and multiple exostoses was found to have a balanced translocation between chromosome 8 and a chromosome X breakpoint located within the gastrin-releasing peptide receptor gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Gastrin-releasing peptide receptor expression in lung cancer.

Archives of pathology & laboratory medicine

Mattei J,Achcar RD,Cano CH,Macedo BR,Meurer L,Batlle BS,Groshong SD,Kulczynski JM,Roesler R,Dal Lago L,Brunetto AT,Schwartsmann G

OPA1-15619 was used in immunohistochemistry to study the expression of GRPR in small cell and non-small cell lung cancer

Wed Jan 01 00:00:00 EST 2014

Expression of gastrin-releasing peptide receptor in patients with cutaneous malignant melanoma.

Clinical and experimental dermatology

Marrone BF,Meurer L,Moretto A,Kleina W,Schwartsmann G

OPA1-15619 was used in Immunohistochemistry-immunofluorescence to determine GRPR expression in biopsy material obtained from patients diagnosed with cutaneous MM.

Tue Oct 01 00:00:00 EDT 2013

Expression of gastrin-releasing peptide receptor in patients with cutaneous malignant melanoma.

Clinical and experimental dermatology

Marrone BF,Meurer L,Moretto A,Kleina W,Schwartsmann G

OPA1-15619 was used in Immunohistochemistry-immunofluorescence to determine GRPR expression in biopsy material obtained from patients diagnosed with cutaneous MM.

Tue Oct 01 00:00:00 EDT 2013

BDNF/TrkB content and interaction with gastrin-releasing peptide receptor blockade in colorectal cancer.

Oncology

Brunetto de Farias C,Rosemberg DB,Heinen TE,Koehler-Santos P,Abujamra AL,Kapczinski F,Brunetto AL,Ashton-Prolla P,Meurer L,Reis Bogo M,Damin DC,Schwartsmann G,Roesler R

OPA1-15619 was used in immunohistochemistry to investigate the involvement of BDNF in colorectal cancer resistance to GRPR treatment

Thu Jun 30 00:00:00 EDT 2011

BDNF and PDE4, but not the GRPR, regulate viability of human medulloblastoma cells.

Journal of molecular neuroscience : MN

Schmidt AL,de Farias CB,Abujamra AL,Kapczinski F,Schwartsmann G,Brunetto AL,Roesler R

OPA1-15619 was used in immunohistochemistry to investigate the influence of BDNF and PDE4 on medulloblastoma cell survival

Mon Mar 01 00:00:00 EST 2010

Stimulation of proliferation of U138-MG glioblastoma cells by gastrin-releasing peptide in combination with agents that enhance cAMP signaling.

Oncology

Farias CB,Lima RC,Lima LO,Flores DG,Meurer L,Brunetto AL,Schwartsmann G,Roesler R

OPA1-15619 was used in immunohistochemistry to study the effect of gastrin-releasing peptide on U138-MG glioblastoma cell proliferation and its mechanism

Mon Oct 06 00:00:00 EDT 2008

Gastrin-releasing peptide receptors regulate proliferation of C6 Glioma cells through a phosphatidylinositol 3-kinase-dependent mechanism.

Current neurovascular research

Flores DG,de Farias CB,Leites J,de Oliveira MS,Lima RC,Tamajusuku AS,Di Leone LP,Meurer L,Brunetto AL,Schwartsmann G,Lenz G,Roesler R

OPA1-15619 was used in immunohistochemistry to investigate the mechanism of glima cell proliferation mediated by gastrin-releasing peptide receptors

Thu May 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: bombesin receptor 2; Gastrin-releasing peptide receptor; GRP receptor; GRP-preferring bombesin receptor; GRP-R

Gene Aliases: BB2; BB2R; GRPR

UniProt ID: (Human) P30550

Entrez Gene ID: (Human) 2925

Molecular Function: transmembrane signal receptor

Function(s)

G-protein coupled receptor signaling pathway cell proliferation regulation of cell proliferation

Process(es)

G-protein coupled peptide receptor activity