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GST Polyclonal Antibody

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Datasheet

Protocols

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GST Antibody in Western Blot (WB) — Western Blot of Goat anti-GST antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: CHO/K1 Lysate (p/n W07-000-357). Lane 4: MDA-MB-231 (p/n W09-001-GK6). Lane 5: A431 Lysate (p/n W09-000-361). Lane 6: Jurkat Lysate (p/n W09-001-370). Lane 7: NIH/3T3 Lysate (p/n W10-000-358). Lane 8: E-coli HCP Control (p/n 000-001-J08). Lane 9: FLAG Positive Control Lysate (p/n W00-001-383 Lane 10: Red Fluorescent Protein (p/n 000-001-379). Lane 11: Green Fluorescent Protein (p/n 000-001-215). Lane 12: Glutathione-S-Transferase Protein Lane 13: Maltose Binding Protein (p/n 000-001-385). Load: 10 µg of lysate or 50ng of purified protein per lane. Primary antibody: GST antibody at 1ug/mL overnight at 4C. Secondary antibody: Peroxidase goat secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 26 kDa for GST.

GST Antibody in Immunoprecipitation (IP) — Immunoprecipitation of anti-GST antibody. Lane 1: T98G cells incubated with GST-Pin1. Lane 2: T98G cells incubated with GST-CDK4/cyclinD1. Lane 3: T98G cells incubated with GST-Pin1 and GST-CDK4/cyclinD1. Immunoprecipitated with pRb antibody. Load: 25 µg per lane. Primary antibody: anti-GST 1:400 for overnight at 4°C. Secondary antibody: IRDye800™ secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.

GST Antibody in ELISA (ELISA) — ELISA results of purified Goat Anti-GST Antibody tested against GST. Each well was coated in 1.0 µg of antigen. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Buffer (p/n MB-060-1000), Substrate (p/n TMB-8000), and Conjugate (Donkey Anti-Goat IgG Antibody HRP at 1:15,000).

Product Details

600-101-200

Applications	Tested Dilution	Publications
Western Blot (WB)	1:5,000-1:10,000	-
Immunohistochemistry (IHC)	1:1,000-1:10,000	-
ELISA (ELISA)	1:20,000-1:50,000	-
Immunoprecipitation (IP)	1:1,000-1:10,000	-

Product Specifications
Species Reactivity	Tag
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Glutathione-S-Transferase [Schistosoma japonicum]
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains	0.01% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Store GST antibody at 4° C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.

Target Information

GST (Glutathione S-transferase) is a 26kDa protein, present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions. Glutathione Stransferase is used to create the GST gene fusion system. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Fusion Proteins offer an important biological assay for direct protein-to-protein interactions. The GST gene fusion system has been introduced into numerous expression vectors.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glutathione-S-Transferase; GST protein; GST Tag

GST Polyclonal Antibody

Product Details

600-101-200

Western Blot (WB)

Immunohistochemistry (IHC)

ELISA (ELISA)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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