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Alomone Labs, LTD
HCN4 Polyclonal Antibody
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FIGURE: 1 / 2
HCN4 Antibody (APC-052-200UL) in IHC
Expression of HCN4 in mouse thalamus - Immunohistochemical staining of mouse thalamus using Anti-HCN4 Antibody (#APC-052). A. HCN4 (red) appears in the neuropil of the lateral nucleus (LN). B. Staining of reticular nucleus (RN) with mouse Anti-Parvalbumin. C. Confocal merge of HCN4 and Parvalbumin images demonstrates separate localization of these proteins in the thalamus.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
APC-052-200UL
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST fusion protein with the sequence HGHLHDSAEERRLIAEGDASPG EDRTPPGLAAEPERP, corresponding to amino acid residues 119-155 of human HCN4, Intracellular, N-terminus
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
0.8 mg/mL once reconstituted
Amount
160 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4, with 1% BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
Target Information
HCN4 is a member of the family of hyperpolarization-activated and cyclic-nucleotide-gated (HCN) channels. HCN currents have been linked to pacemaker activity in the heart and brain, resting potential control, as well as neuronal plasticity. It has been shown that HCN4 channels function as receptors for sour taste, and are associated with pacemaker potential generation in the sinoatrial node.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain cyclic nucleotide-gated channel 3; hyperpolarization activated cyclic nucleotide-gated cation channel 4; hyperpolarization-activated, cyclic nucleotide-gated K+ 3; hyperpolarization-activated, cyclic nucleotide-gated K+ 4
Gene Aliases: Bcng3; BRGDA8; EIG18; Hcn3; SSS2
UniProt ID: (Human) Q9Y3Q4, (Rat) Q9JKA7, (Mouse) O70507
Entrez Gene ID: (Human) 10021, (Rat) 59266, (Mouse) 330953
ion channel activity
intracellular cAMP activated cation channel activity
voltage-gated sodium channel activity
voltage-gated potassium channel activity
sodium channel activity
protein binding
identical protein binding
voltage-gated potassium channel activity involved in SA node cell action potential depolarization
potassium channel activity
in utero embryonic development
regulation of heart rate
regulation of membrane depolarization
ion transport
cation transport
potassium ion transport
regulation of heart contraction
sodium ion transmembrane transport
regulation of membrane potential
modulation of synaptic transmission
transmembrane transport
regulation of cardiac muscle contraction
cellular response to cAMP
cellular response to cGMP
potassium ion transmembrane transport
SA node cell action potential
membrane depolarization during SA node cell action potential
regulation of heart rate by cardiac conduction
sodium ion import across plasma membrane
regulation of cardiac muscle cell action potential involved in regulation of contraction
cellular response to aldosterone
potassium ion import across plasma membrane
sinoatrial node development
muscle contraction
blood circulation
membrane depolarization during cardiac muscle cell action potential
regulation of SA node cell action potential
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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