Proteintech

HRAS Polyclonal Antibody

Product Image Gallery

HRAS Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells using 18295-1-AP (HRAS-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

HRAS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate hyperplasia slide using 18295-1-AP (HRAS-Specific Antibody) at dilution of 1:50.

HRAS Antibody in Western Blot (WB) — SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18295-1-AP (HRAS-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

HRAS Antibody in Flow Cytometry (Flow) — 1X10^6 HeLa cells were stained with 1ug HRAS-Specific antibody (18295-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

HRAS Antibody in Immunoprecipitation (IP) — IP result of anti-HRAS-Specific (IP:18295-1-AP, 4ug; Detection:18295-1-AP 1:3000) with HEK-293 cells lysate 3040 ug.

Product Details

Tested Applications

Dilution

Flow Cytometry (Flow)

1 µg/1x10^6 cells

Immunocytochemistry (ICC)

1:10-1:100

Immunofluorescence (IF)

1:10-1:100

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:500-1:2000

Published Applications

Immunofluorescence (IF)

See 1 publication below

Western Blot (WB)

See 10 publications below

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide

Conjugate

Unconjugated

Form

Liquid

Concentration

0.31 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

The antibody is specific to HRAS and does not recognize NRAS and KRAS.

Target Information

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C BAS/HAS; C H RAS; C HA RAS1; c-H-ras; c-Ha-ras p21 protein; c-Ha-ras transgene; c-has/bas p21 protein; c-ras-Ki-2 activated oncogene; CTLO; GTP- and GDP-binding peptide B; GTPase HRas; GTPase HRas, N-terminally processed; H Ras 1; H RASIDX; H-ras 1 protein; H-Ras-1; Ha Ras; Ha-Ras; Ha-Ras1 proto-oncoprotein; HAMSV; Harvey ras1 protein; Harvey rat sarcoma viral (v-Ha-ras) oncogene homolog; Harvey rat sarcoma viral oncogene homolog; Harvey rat sarcoma viral oncoprotein; Harvey rat sarcoma virus oncogene 1; HRAS; HRAS1; K RAS; p19 H-RasIDX protein; p21ras; Ras family small GTP binding protein H-Ras; RASH1; transformation gene: oncogene HAMSV; Transforming protein p21; v-Ha-ras Harvey rat sarcoma viral oncogene homolog

Gene Aliases: C-BAS/HAS; C-H-RAS; c-Ha-ras; C-HA-RAS1; c-rasHa; CTLO; H-ras; H-RASIDX; Ha-ras; HAMSV; Harvey-ras; HRAS; Hras-1; HRAS1; Kras2; p21ras; ras; RASH1

UniProt ID: (Human) P01112, (Mouse) Q61411, (Rat) P20171

Entrez Gene ID: (Human) 3265, (Mouse) 15461, (Rat) 293621

Molecular Function: G-protein enzyme modulator small GTPase