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Invitrogen
IFN gamma Polyclonal Antibody
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FIGURE: 1 / 2
IFN gamma Antibody (PA5-48107) in Neu
Neutralization of IFN gamma in HeLa human cervical epithelial carcinoma cell line. Samples were incubated in IFN gamma polyclonal antibody (Product # PA5-48107) using a dilution of 200 ng/mL. Recombinant Rhesus Macaque IFN-γ reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rhesus Macaque IFN-γ is neutralized (green line) by increasing c... View More
Product Details
PA5-48107
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rhesus monkey
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived recombinant rhesus macaque IFN- gamma Gln24-Gln165
Conjugate
Form
Lyophilized
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody detects rhesus macaque IFN-gamma in ELISAs and Western blots. In sandwich ELISAs, less than 30% cross-reactivity with recombinant human IFN- gamma is observed and less than 0.1% cross-reactivity with recombinant mouse IFN- gamma, recombinant rat IFN- gamma, recombinant porcine IFN- gamma, recombinant canine IFN- gamma, recombinant cotton rat IFN- gamma, and recombinant feline IFN- gamma is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines. IFN gamma is a dimeric protein consisting of two 146 amino acid subunits. IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa. On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation. The biological activity of the IFN gamma homodimer is highly species specific. Human IFN gamma does not show cross-reactivity with mouse. IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes. IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines. Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway. IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma. IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. IFN gamma gene mutations are associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; H-IFN-g; IFN γ; IFN-gamma; IFN-y; IFNγ; Interferon; Interferon gamma; Interferon y; Interferon γ; Interferonγ; M-IFN-g; R-IFN-g
Gene Aliases: EGK_03901; IFN-gamma; IFNG
UniProt ID: (Rhesus monkey) P63310
Entrez Gene ID: (Rhesus monkey) 574282
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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