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|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant human IL-13|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ELISA (ELISA)||See 6 publications below|
P130E targets IL-13 in ELISA, and WB applications and shows reactivity with Human samples.
The P130E immunogen is recombinant human IL-13.
P130E detects IL-13 which has a predicted molecular weight of approximately 13 kDa.
The P130E IL13 polyclonal antibody has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M130B (biotinylated conjugate of clone 5A2) Typical dilutions for sandwich ELISA include 1 µg/ml for coating and 0.125 - 0.25 µg/ml for detection.
Antibody P130E and biotinylated antibody M130B (clone 5A2) have successfully been used in combination with recombinant IL13 protein SIL13 in ELISA applications.
This gene encodes an immunoregulatory cytokine produced primarily by activated Th2 cells. This cytokine is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The CD226/CD155 interaction regulates the proinflammatory (Th1/Th17)/anti-inflammatory (Th2) balance in humans.
P130E was used in ELISA to study the role of the interaction between CD226 and CD155 in regulating the human T-cell pro-inflammatory/anti-inflammatory balance
|Lozano E,Joller N,Cao Y,Kuchroo VK,Hafler DA||Journal of immunology (Baltimore, Md. : 1950) (191:3673)||2013|
Fibroblasts stimulated via HLA-II molecules produce prostaglandin E? and regulate cytokine production from helper T cells.
P130E was used in ELISA to study the role of HLA-DQ molecules in Th-cell responses
|Kato-Kogoe N,Ohyama H,Nishimura F,Meguro M,Yoshizawa S,Okada Y,Nakasho K,Yamanegi K,Yamada N,Hata M,Higashi T,Terada N,Matsushita S||Laboratory investigation; a journal of technical methods and pathology (90:1747)||2010|
Can prenatal malaria exposure produce an immune tolerant phenotype? A prospective birth cohort study in Kenya.
P130E was used in ELISA to study the effect of the prenatal malaria exposure on immune tolerance
|Malhotra I,Dent A,Mungai P,Wamachi A,Ouma JH,Narum DL,Muchiri E,Tisch DJ,King CL||PLoS medicine (6:null)||2009|
Modulation of the infant immune responses by the first pertussis vaccine administrations.
P130E was used in ELISA to study the interleukin 13 production in infants with different pertussis-tetanus-diphtheria-poliomyelitis vaccines
|Mascart F,Hainaut M,Peltier A,Verscheure V,Levy J,Locht C||Vaccine (25:391)||2007|
Distinct Th1- and Th2-Type prenatal cytokine responses to Plasmodium falciparum erythrocyte invasion ligands.
P130E was used in ELISA to characterize the prenatal immune responses against Plasmodium falciparum erythrocyte invasion ligands
|Malhotra I,Mungai P,Muchiri E,Ouma J,Sharma S,Kazura JW,King CL||Infection and immunity (73:3462)||2005|
Age-dependent cellular immune responses to Plasmodium vivax Duffy binding protein in humans.
P130E was used in ELISA to investigate the role of plasmodium vivax duffy binding protein
|Xainli J,Baisor M,Kastens W,Bockarie M,Adams JH,King CL||Journal of immunology (Baltimore, Md. : 1950) (169:3200)||2002|