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ITCH Antibody (PA5-98685) in ICC/IF
Immunofluorescent analysis of ITCH in HepG2 cells using a ITCH polyclonal antibody (Product # PA5-98685) at a dilution of 1:133. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.
Product Details
PA5-98685
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant Human E3 ubiquitin-protein ligase Itchy homolog protein (29-303AA)
Conjugate
Form
Liquid
Concentration
4.26 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.03% ProClin 300
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Wet ice
RRID
AB_2813298
Target Information
Atrophin-1 contains a polyglutamine repeat, expansion of which is responsible for dentatorubral and pallidoluysian atrophy. The protein encoded by this gene interacts with atrophin-1. This encoded protein is a closely related member of the NEDD4-like protein family. This family of proteins are E3 ubiquitin-ligase molecules and regulate key trafficking decisions, including targeting of proteins to proteosomes or lysosomes. This encoded protein contains four tandem WW domains and a HECT domain. It can act as a transcriptional corepressor of p45/NFE2 and may participate in the regulation of immune responses by modifying Notch-mediated signaling. It is highly similar to the mouse Itch protein, which has been implicated in the regulation and differentiation of erythroid and lymphoid cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AIP4; atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; E3 ubiquitin-protein ligase Itchy homolog; HECT-type E3 ubiquitin transferase Itchy homolog; Itch; itchy E3 ubiquitin protein ligase homolog; NAPP1; NFE2-associated polypeptide 1
Gene Aliases: ADMFD; AIF4; AIP4; ITCH; NAPP1
UniProt ID: (Human) Q96J02
Entrez Gene ID: (Human) 83737
regulation of cell growth
positive regulation of T cell anergy
ubiquitin-dependent protein catabolic process
apoptotic process
inflammatory response
Notch signaling pathway
protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
negative regulation of type I interferon production
protein K29-linked ubiquitination
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
innate immune response
positive regulation of protein catabolic process
negative regulation of JNK cascade
negative regulation of alpha-beta T cell proliferation
viral entry into host cell
negative regulation of defense response to virus
defense response to virus
nucleotide-binding oligomerization domain containing signaling pathway
protein K63-linked ubiquitination
protein K48-linked ubiquitination
regulation of protein deubiquitination
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