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Invitrogen
Integrin beta 7 Chimeric Recombinant Rabbit Monoclonal Antibody (FIB27)
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FIGURE: 1 / 1
Integrin beta 7 Chimeric Antibody (MA5-48015) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Integrin beta 7 in human peripheral blood monocytes (PBMs) using Integrin beta 7 Chimeric Monoclonal Antibody (Product # MA5-48015). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with Integrin beta 7 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing membrane staining. ... View More
Product Details
MA5-48015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
FIB27
Immunogen
FIB27 was prepared by immunizing rats with TK1 cells.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Specificity: FIB27 binds specifically to both human and mouse integrin beta-7 at epitope region Dl on which is involved in all a4b7-mediated adhesion events. Does not compete with DAKT32 for binding. FIB27 blocks LS722-induced TK1 cell aggregation (mAb LS722 is an activating anti-a4B7 antibody and induces aggregation via an a4B7-dependent pathway).
Target Information
Integrin beta 7 associates with integrin alpha 4 (CD49d) to form the alpha 4 beta 7 integrin LPAM-1, expressed on intraepithelial lymphocytes. It also associates with alpha E (CD103) to form the alpha E beta 7 integrin HML-1, expressed on T cells adjacent to mucosal epithelium and intraepithelial lymphocytes. Ligands for LPAM-1 include VCAM-1 (CD106), MAdCAM-1 and fibronectin, while the ligand of HML-1 is E-cadherin (CD324). Integrin beta 7 plays an important role in the adhesion of leukocytes to endothelial cells promoting the transmigration of leukocytes to extravascular spaces during the inflammatory response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta-7; Integrin beta-P; M290 IEL antigen; unnamed protein product
Gene Aliases: ITGB7; Ly69
UniProt ID: (Human) P26010, (Mouse) P26011
Entrez Gene ID: (Human) 3695, (Mouse) 16421
immune response in gut-associated lymphoid tissue
cell-matrix adhesion involved in ameboidal cell migration
cell adhesion
cell-matrix adhesion
integrin-mediated signaling pathway
cell adhesion mediated by integrin
heterotypic cell-cell adhesion
substrate adhesion-dependent cell spreading
receptor clustering
viral entry into host cell
leukocyte migration
leukocyte tethering or rolling
T cell migration
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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