|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A 17 amino acid peptide near the carboxy terminus of human LRRTM2.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2.5 µg/ml|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
A suggested positive control is SK-N-SH cell lysate.
PA5-21097 can be used with blocking peptide PEP-1211.
The Leucine-rich repeat transmembrane neuronal proteins (LRRTMs) are differentially expressed in the nervous system and were recently found to instruct presynaptic and mediate postsynaptic glutamatergic differentiation, with LRRTM1 and LRRTM2 most potent at inducing presynaptic differentiation. Each LRRTM protein is a type I transmembrane containing ten extracellular leucine-rich repeats and a short intracellular tail and has a developmentally regulated pattern distinct from all others. LRRTM2 interacts with PSD-95 and regulates the surface expression of AMPA receptors. LRRTM2 also functions as a neurexin ligand, binding both Neurexin 1-alpha and -beta, suggesting that LRRTM2-Neurexin1 interaction plays a critical role in regulatory excitatory synapse development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A model for regulation by SynGAP-¿1 of binding of synaptic proteins to PDZ-domain 'Slots' in the postsynaptic density.
PA5-21097 was used in western blot demonstrate that synGAP-alpha1 regulates postsynaptic density composition by restricting binding to the PDZ domains of PSD-95
|Walkup WG,Mastro TL,Schenker LT,Vielmetter J,Hu R,Iancu A,Reghunathan M,Bannon BD,Kennedy MB||eLife (5:null)||2016|