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Invitrogen
Skeletal Muscle Actin Monoclonal Antibody (5C5.F8.C7)
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FIGURE: 1 / 4
Skeletal Muscle Actin Antibody (MA1-37021) in ICC/IF
Immunofluorescence analysis of ACTA1 was performed using 70% confluent log phase C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Skeletal Muscle Actin Monoclonal Antibody (Product # MA1-37021) at 1:100 dilution in 0.1% BSA, incubated at 4 degree... View More
Product Details
MA1-37021
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human,
Mouse,
Rabbit
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
5C5.F8.C7
Immunogen
N-terminus decapeptide of alpha skeletal muscle isoform of actin
Conjugate
Form
Liquid
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is skeletal muscle tissue.
Target Information
The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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