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Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8)
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FIGURE: 1 / 16
Phospho-RNA pol II CTD (Ser5) Antibody (MA1-46093) in ICC/IF
Immunofluorescence analysis of NPhospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8) was performed using 70% confluent log phase HCT 116 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Phospho-RNA pol II CTD (Ser5) Monoclonal Antibody (4H8) (Pr... View More
Product Details
MA1-46093
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Yeast
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4H8
Immunogen
Chemically synthesized phospho-ser 5 YSPTSpPS (Human).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-46093 reacts with DNA-directed RNA Polymerase II Largest Subunit in human, yeast and mouse samples. Expected to cross-react with Drosophilia melanogaster, hamster, S. pombe, and Arabidopsis thaliana due to sequence homology.
MA1-46093 has been successfully used in Western Blot, Chromatin Immunoprecipitation, ELISA, Immunocytochemistry/Immunofluorescence, IHC (P) and Immunoprecipitation applications.
The MA1-46093 immunogen is 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser 5 YSPTSpPS (Human). This antibody detects both the phosphorylated and non-phosphorylated forms.
Target Information
RNA polymerase II is the enzyme responsible for the synthesis of mRNA and many non-coding RNAs. POLR2A encodes RNA polymerase II subunit B1 (RPB1), the largest subunit of RNA polymerase II. The C-terminal domain (CTD) of RPB1 contains tandem heptapeptide repeats with the consensus sequence YSPTSPS. Phosphorylation and other modifications within this sequence influence the recruitment of factors that regulate the transcription cycle. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the CTD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; 3'-5' exoribonuclease; DNA polymerase epsilon catalytic subunit A; DNA polymerase II subunit A; DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220 kd subunit; DNA-directed RNA polymerase II subunit A; DNA-directed RNA polymerase II subunit RPB1; DNA-directed RNA polymerase III largest subunit; EC 2.7.7.6; MGC75453; Pol II S2p; polymerase (RNA) II (DNA directed) polypeptide A, 220kDa; polymerase (RNA) II subunit A; POLYMERASE II; RNA; RNA POL2; RNA polymerase II; RNA polymerase II 1; RNA polymerase II C-terminal domain; RNA polymerase II carboxy-terminal domain; RNA polymerase II subunit B1; RNA-directed RNA polymerase II subunit RPB1; RP02; RPB220; RPO21; SUA8; unnamed protein product
Gene Aliases: 220kDa; DUN2; hRPB220; hsRPB1; N0825; NEDHIB; POL2; POLR2; POLR2A; POLRA; RPB1; RPBh1; Rpii215; RpIILS; RPO2; Rpo2-1; RPOL2; YNL262W
UniProt ID: (Baker's yeast) P21951, (Human) P24928, (Mouse) P08775
Entrez Gene ID: (Baker's yeast) 855459, (Human) 5430, (Mouse) 20020
nucleotide binding
nucleic acid binding
DNA binding
double-stranded DNA binding
single-stranded DNA binding
mRNA binding
DNA-directed DNA polymerase activity
zinc ion binding
single-stranded DNA 3'-5' exodeoxyribonuclease activity
transferase activity
nucleotidyltransferase activity
hydrolase activity
SUMO binding
metal ion binding
iron-sulfur cluster binding
4 iron, 4 sulfur cluster binding
magnesium ion binding
RNA binding
DNA-directed RNA polymerase activity
RNA-directed RNA polymerase activity
protein binding
kinase binding
ubiquitin protein ligase binding
RNA polymerase activity
microfibril binding
promoter-specific chromatin binding
core promoter binding
RNA polymerase II activity
poly(A) RNA binding
DNA metabolism protein
DNA-directed RNA polymerase
mitotic cell cycle
DNA replication
DNA-dependent DNA replication
leading strand elongation
DNA repair
base-excision repair
base-excision repair, gap-filling
nucleotide-excision repair, DNA gap filling
double-strand break repair
double-strand break repair via nonhomologous end joining
mitotic sister chromatid cohesion
intra-S DNA damage checkpoint
mitotic DNA replication checkpoint
gene conversion
error-prone translesion synthesis
DNA replication proofreading
nucleic acid phosphodiester bond hydrolysis
transcription, RNA-templated
transcription, DNA-templated
DNA-templated transcription, termination
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
transcription elongation from RNA polymerase II promoter
positive regulation of gene expression
positive regulation of RNA splicing
mRNA transcription from RNA polymerase II promoter
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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