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Invitrogen
SUMO1 Monoclonal Antibody (T.243.0)
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FIGURE: 1 / 7
SUMO1 Antibody (MA5-14877) in ICC/IF
Immunofluorescence analysis of SUMO1 (Small ubiquitin-related modifier 1) was performed using 80% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SUMO1 Monoclonal Antibody (T.243.0) (Product # MA5-14877) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, A... View More
Product Details
MA5-14877
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
T.243.0
Immunogen
Synthetic peptide from the amino terminus of human SUMO-1
Conjugate
Form
Liquid
Concentration
23 µg/mL
Amount
2.3 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG4494-PA; GAP modifying protein 1; GAP-modifying protein 1; GMP1; LD07775p; Mutations: 288:OK; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; Sentrin; Sentrin-1; similar to ubiquitin and to yeast Smt3p (suppressor of MIF2); Method: conceptual translation supplied by author; Small ubiquitin-related modifier 1; small ubiquitin-related modifier-1; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; Smt3C; SUMO-1; SUMO-1 related peptidase; Ubiquitin-homology domain protein PIC1; ubiquitin-like 1; ubiquitin-like 1 (sentrin); ubiquitin-like protein; Ubiquitin-like protein SMT3C; Ubiquitin-like protein UBL1; UBL1; unnamed protein product
Gene Aliases: DAP1; GMP1; OFC10; OK/SW-cl.43; PIC1; SENP2; SENTRIN; SMT3; SMT3C; SMT3H3; SMTP3; SUMO-1; SUMO1; UBL1
UniProt ID: (Human) P63165, (Mouse) P63166, (Rat) Q5I0H3
Entrez Gene ID: (Human) 7341, (Mouse) 22218, (Rat) 301442
RNA binding
protein binding
transcription factor binding
potassium channel regulator activity
enzyme binding
protein tag
ubiquitin protein ligase binding
small protein activating enzyme binding
ubiquitin-like protein ligase binding
transporter activator activity
ubiquitin-specific protease binding
SUMO transferase activity
ion channel binding
poly(A) RNA binding
DNA repair
regulation of transcription, DNA-templated
negative regulation of transcription by transcription factor localization
protein sumoylation
PML body organization
positive regulation of protein complex assembly
regulation of protein stability
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of protein localization
cellular response to heat
negative regulation of protein import into nucleus
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of action potential
negative regulation of transcription, DNA-templated
protein stabilization
palate development
cellular response to cadmium ion
regulation of cardiac muscle cell contraction
protein localization to nuclear pore
negative regulation of potassium ion transmembrane transporter activity
negative regulation of delayed rectifier potassium channel activity
regulation of calcium ion transmembrane transport
positive regulation of calcium-transporting ATPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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