Immunohistochemistry analysis of HSPB8 in paraffin-embedded mouse back skin. Samples were incubated with HSPB8 monoclonal antibody (Product # MA5-27706) using a dilution of 1:100 (1 hour at RT) and followed by FITC Goat Anti-Rabbit (green) at a dilution of 1:50 (1 hour at RT).

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Immunogen image

Immunogen Image HSPB8 Monoclonal Antibody (3C12-H11)

Product Details

MA5-27706

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100

Immunocytochemistry (ICC/IF)

Assay-Dependent

ELISA (ELISA)

Assay-Dependent

Product Specifications

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

3C12-H11

Immunogen

Full-length his-tagged human recombinant HSP22

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2735223

Product Specific Information

1 µg/mL of MA5-27706 was sufficient for detection of HSP22 in 20 µg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 22kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

Target Information

The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The expression of this gene in induced by estrogen in estrogen receptor-positive breast cancer cells, and this protein also functions as a chaperone in association with Bag3, a stimulator of macroautophagy. Thus, this gene appears to be involved in regulation of cell proliferation, apoptosis, and carcinogenesis, and mutations in this gene have been associated with different neuromuscular diseases, including Charcot-Marie-Tooth disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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HSPB8 Monoclonal Antibody (3C12-H11)

HSPB8 Antibody (MA5-27706) in IHC (P)