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Invitrogen
CD43 Monoclonal Antibody (MEM-59), Alexa Fluor™ 700
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-28597
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-59
Immunogen
Human T lymphocytes.
Conjugate
Alexa Fluor™ 700
Alexa Fluor™ 700
Alexa Fluor™ 700
Excitation/Emission Max
696/719 nm
View spectra
Form
Liquid
Purification
Size-exclusion chromatography
Storage buffer
TBS with 0.2% BSA
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes a neuraminidase-sensitive extracellular epitope on CD43 (Leukosialin), a 95-135 kDa type I transmembrane glycoprotein (mucin-type) which is involved in lymphocyte activation. CD43 is expressed by platelets and at high levels on the surface of all leukocytes; it is negative on resting B lymphocytes and erythrocytes.
Recommended dilution is for 100 µL of whole blood or 1x10^6 cells in suspension.
Target Information
CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: galactoglycoprotein; leukocyte sialoglycoprotein; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115; sialophorin precursor; unnamed protein product
Gene Aliases: CD43; GALGP; GPL115; LEU-22; LSN
UniProt ID: (Human) P16150
Entrez Gene ID: (Human) 6693
T-helper 1 cell lineage commitment
chemotaxis
immune response
cellular defense response
negative regulation of cell adhesion
establishment or maintenance of cell polarity
signal transduction
cell surface receptor signaling pathway
positive regulation of tumor necrosis factor production
negative regulation of T cell proliferation
defense response to bacterium
regulation of immune response
regulation of T cell activation
leukocyte tethering or rolling
regulation of T cell migration
positive regulation of T cell migration
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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