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Invitrogen
Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (SA35-03)
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FIGURE: 1 / 9
Cytokeratin 20 Antibody (MA5-31979) in ICC/IF
Immunofluorescence analysis of KRT20 was performed using HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 20 Recombinant Rabbit Monoclonal Antibody (Product # MA5-31979) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and t... View More
Product Details
MA5-31979
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SA35-03
Immunogen
Synthetic peptide within Human Cytokeratin 20 aa 375-424.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CK-20; CK-21; cytokeratin 20; cytokeratin 21; Cytokeratin-20; Cytokeratin-21; K20; keratin 20, type I; Keratin, type I cytoskeletal 20; Keratin-20; MGC35423; Protein IT; unnamed protein product
Gene Aliases: CD20; CK-20; CK20; K20; KRT20; KRT21
UniProt ID: (Human) P35900, (Rat) P25030
Entrez Gene ID: (Human) 54474, (Rat) 286912
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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