Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87 kDa ENaC beta protein. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Samples were incubated with ENaC beta monoclonal antibody (Product # MA5-45357) at 1:1,000. Predicted/Observed Size: ~87 kDa.

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image SCNN1B Monoclonal Antibody (16E4), PerCP

Product Details

MA5-45357

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Product Specifications

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

16E4

Immunogen

Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)

3D Epitope / Immunogen

Conjugate

PerCP PerCP PerCP

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

100 µg

Storage conditions

4°C

Ambient (domestic); Wet ice (international)

AB_2931811

Product Specific Information

A 1:1,000 dilution of MA5-45357 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 87kDa.

Target Information

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. Heterotetramer of two alpha, one beta and one gamma subunit. A delta subunit can replace the alpha subunit. Interacts with the WW domains of NEDD4, NEDD4L, WWP1 and WWP2. Defects in SCNN1B are a cause of autosomal recessive pseudohypoaldosteronism type 1 (PHA1) [MIM:264350]. PHA1 is a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. There are 2 forms of PHA1: the autosomal recessive form that is severe, and the dominant form which is more milder and due to defects in mineralocorticoid receptor. Autosomal recessive PHA1 is characterized by an often fulminant presentation in the neonatal period with dehydration, hyponatraemia, hyperkalaemia, metabolic acidosis, failure to thrive and weight loss. Defects in SCNN1B are a cause of Liddle syndrome. It is an autosomal dominant disorder characterized by pseudoaldosteronism and hypertension associated with hypokalemic alkalosis. The disease is caused by constitutive activation of the renal epithelial sodium channel.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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SCNN1B Monoclonal Antibody (16E4), PerCP

SCNN1B Antibody (MA5-45357) in WB