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Invitrogen
TRPV3 Monoclonal Antibody (N15/4), PE
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FIGURE: 1 / 4
TRPV3 Antibody (MA5-45430) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with TrpV3 monoclonal antibody (Product # MA5-45430) at 1:100 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 antibody (D) Composite.
Product Details
MA5-45430
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N15/4
Immunogen
Synthetic peptide amino acids 774-791 (C-terminus) of rat TrpV3
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45430 was sufficient for detection of TrpV3 in 10 µg of COS-1 cell lysate transiently transfected with TrpV3 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 70kDa.
Target Information
TRPV3 belongs to a family of nonselective cation channels that function in a variety of processes, including temperature sensation and vasoregulation. The thermosensitive members of this family are expressed in subsets of sensory neurons that terminate in the skin, and are activated at distinct physiological temperatures. This channel is activated at temperatures between 22 and 40 degrees C. This gene lies in close proximity to another family member (TRPV1) gene on chromosome 17, and the two encoded proteins are thought to associate with each other to form heteromeric channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat sensitive channel TRPV3; ion channel; MGC124324; MGC124325; non-hair; Transient receptor potential cation channel subfamily V member 3; TRP ion channel TRPV3; TrpV3; unnamed protein product; Vanilloid receptor-like 3; vanilloid receptor-related osmotically activated channel protein; VRL 3; VRL-3
Gene Aliases: 1110036I10Rik; AI644701; FNEPPK2; Nh; OLMS; OLMS1; TRPV3; VRL3
UniProt ID: (Human) Q8NET8, (Mouse) Q8K424
Entrez Gene ID: (Human) 162514, (Mouse) 246788, (Rat) 497948
ion transport
calcium ion transport
actin filament organization
osmosensory signaling pathway
response to temperature stimulus
ion transmembrane transport
sodium ion transmembrane transport
negative regulation of hair cycle
transmembrane transport
calcium ion transmembrane transport
positive regulation of calcium ion import
calcium ion import across plasma membrane
transport
cation transmembrane transport
response to heat
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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