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Invitrogen
GABRA1 Monoclonal Antibody (N95/35), APC
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FIGURE: 1 / 3
GABRA1 Antibody (MA5-45485) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human neuroblastoma cells. Fixation involved 4% PFA for 15 min. Samples were incubated with GABA-A Receptor Alpha-1 monoclonal antibody (Product # MA5-45485) at 1:50 for overnight at 4°C with slow rocking, followed by AlexaFluor 488 at 1:1,000 for 1 hour at RT. Counterstain used was Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GABA-A Receptor ... View More
Product Details
MA5-45485
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N95/35
Immunogen
Fusion protein amino acids 355-394 of human GABA-A-R-Alpha1
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45485 was sufficient for detection of alpha1 GABA receptor in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 55kDa. No cross-reactivity against GABA-A-R-Alpha 2 or -Alpha3.
Target Information
Gamma -aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA Receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date aix alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified. Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-R sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor. Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GABA(A) receptor subunit alpha-1; GABA(A) receptor, alpha 1; GABAA alpha 1; GABAAR alpha1; GABAAR subunit alpha-1; GABRA 1; gamma-aminobutyric acid; gamma-aminobutyric acid A receptor, alpha 1; gamma-aminobutyric acid (GABA) A receptor, alpha 1; gamma-aminobutyric acid (GABA-A) receptor, subunit alpha 1; gamma-aminobutyric acid A receptor, alpha 1; Gamma-aminobutyric acid receptor subunit alpha-1; gamma-aminobutyric acid type A receptor alpha1 subunit; unnamed protein product
Gene Aliases: DEE19; ECA4; EIEE19; EJM; EJM5; Gabra-1; GABRA1
UniProt ID: (Human) P14867, (Rat) P62813, (Mouse) P62812
Entrez Gene ID: (Human) 2554, (Rat) 29705, (Mouse) 14394
transmembrane signaling receptor activity
GABA-A receptor activity
ion channel activity
extracellular ligand-gated ion channel activity
chloride channel activity
benzodiazepine receptor activity
GABA receptor activity
GABA-gated chloride ion channel activity
signaling receptor activity
transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential
inhibitory extracellular ligand-gated ion channel activity
protein binding
drug binding
ligand-gated ion channel
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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