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MAP1B Polyclonal Antibody

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MAP1B Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells using 21633-1-AP (MAP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MAP1B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded mouse cerebellum tissue slide using 21633-1-AP (MAP1B antibody) at dilution of 1:400 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

MAP1B Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 21633-1-AP (MAP1B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

MAP1B Antibody in Western Blot (WB) — Mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 21633-1-AP (MAP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

MAP1B Antibody in Flow Cytometry (Flow) — 1X10^6 SH-SY5Y cells were stained with 0.2ug MAP1B antibody (21633-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

Product Details

21633-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 4 publications 4 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:800	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:10-1:100	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MAP1B Fusion Protein Ag16255 (414-567 aa encoded by BC141853) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.49 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: DSRESLKPA AKPLPSKSVR KESKEETPEV TKVNHVEKPP KVESKEKVMV KKDKPVKTET KPSVTEKEVP SKEEPSPVKA EVAEKQATDV KPKAAKEKTV KKETKVKPED KKEEKEKPKK EVAKKEDKTP IKKEEKPKKE EVKKEVKKEI KKEEK (414-567 aa encoded by BC141853)

Target Information

The coenzyme NAD and its derivatives are involved in hundreds of metabolic redox reactions and are utilized in protein ADP-ribosylation, histone deacetylation, and in some Ca(2+) signaling pathways. NMNAT.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

MAP1B-LC1 prevents autophagosome formation by linking syntaxin 17 to microtubules.

EMBO reports

Arasaki K,Nagashima H,Kurosawa Y,Kimura H,Nishida N,Dohmae N,Yamamoto A,Yanagi S,Wakana Y,Inoue H,Tagaya M

Wed Aug 01 00:00:00 EDT 2018

eEF2K/eEF2 Pathway Controls the Excitation/Inhibition Balance and Susceptibility to Epileptic Seizures.

Cerebral cortex (New York, N.Y. : 1991)

Heise C,Taha E,Murru L,Ponzoni L,Cattaneo A,Guarnieri FC,Montani C,Mossa A,Vezzoli E,Ippolito G,Zapata J,Barrera I,Ryazanov AG,Cook J,Poe M,Stephen MR,Kopanitsa M,Benfante R,Rusconi F,Braida D,Francolini M,Proud CG,Valtorta F,Passafaro M,Sala M,Bachi A,Verpelli C,Rosenblum K,Sala C

Wed Mar 01 00:00:00 EST 2017

Depletion of JMJD5 sensitizes tumor cells to microtubule-destabilizing agents by altering microtubule stability.

Cell cycle (Georgetown, Tex.)

Wu J,He Z,Wang DL,Sun FL

Tue Nov 01 00:00:00 EDT 2016

GIT1 enhances neurite outgrowth by stimulating microtubule assembly.

Neural regeneration research

Li YS,Qin LX,Liu J,Xia WL,Li JP,Shen HL,Gao WQ

Tue Mar 01 00:00:00 EST 2016

Bioinformatics

Protein Aliases: DKFZp686E1099; DKFZp686F1345; FLJ38954; MAP-1B; MAP1 light chain LC1; MAP1(X); MAP1.2; MAP1B heavy chain; Microtubule-associated protein 1B; Microtubule-associated protein 1B (MAP 1B) (MAP1.2) (MAP1(X)); microtubule-associated protein 5; protein phosphatase 1, regulatory subunit 102

Gene Aliases: A230055D22; AI843217; FUTSCH; LC1; MAP1B; MAP5; Mtap-5; Mtap1b; Mtap5; PPP1R102

UniProt ID: (Human) P46821, (Mouse) P14873

Entrez Gene ID: (Human) 4131, (Mouse) 17755

Molecular Function: non-motor microtubule binding protein