Proteintech

MMP1 Polyclonal Antibody

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MMP1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using MMP1 antibody 10371-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

MMP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using 10371-2-AP ( MMP1 Antibody) at dilution of 1:200 (under 40x lens).

MMP1 Antibody in Western Blot (WB) — Mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10371-2-AP (MMP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

MMP1 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug MMP1 antibody (10371-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1000.

MMP1 Antibody in Immunoprecipitation (IP) — IP result of anti-MMP1 (IP:10371-2-AP, 4ug; Detection:10371-2-AP 1:1000) with A549 cells lysate 1200ug.

Product Details

10371-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 24 publications 24 publications
Immunohistochemistry (IHC)	-	View 7 publications 7 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 3 publications 3 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rabbit, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	MMP1 Fusion Protein Ag0547 (1-200 aa encoded by BC013875) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.38 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MHSFPPLLL LLFWGVVSHS FPATLETQEQ DVDLVQKYLE KYYNLKNDGR QVEKRRNSGP VVEKLKQMQE FFGLKVTGKP DAETLKVMKQ PRCGVPDVAQ FVLTEGNPRW EQTHLTYRIE NYTPDLPRAD VDHAIEKAFQ LWSNVTPLTF TKVSEGQADI MISFVRGDHR DNSPFDGPGG NLAHAFQPGP GIGGDAHFDE D (1-200 aa encoded by BC013875)

Target Information

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Alternative splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model.

Evidence-based complementary and alternative medicine : eCAM

Zeng L,Xiao CZ,Deng ZT,Li RH

Thu Oct 01 00:00:00 EDT 2020

MiR-202-3p regulates interleukin-1β-induced expression of matrix metalloproteinase 1 in human nucleus pulposus.

Gene

Shi C,Wu L,Lin W,Cai Y,Zhang Y,Hu B,Gao R,Im HJ,Yuan W,Ye X,van Wijnen AJ

Fri Mar 01 00:00:00 EST 2019

MiR-202-3p regulates interleukin-1β-induced expression of matrix metalloproteinase 1 in human nucleus pulposus.

Gene

Shi C,Wu L,Lin W,Cai Y,Zhang Y,Hu B,Gao R,Im HJ,Yuan W,Ye X,van Wijnen AJ

Fri Mar 01 00:00:00 EST 2019

Expression variations and clinical significance of MMP-1, MMP-2 and inflammatory factors in serum of patients with deep venous thrombosis of lower extremity.

Experimental and therapeutic medicine

Zhang T,Li Q,Wang L,Li G

Tue Jan 01 00:00:00 EST 2019

Caffeic acid protects against IL-1β-induced inflammatory responses and cartilage degradation in articular chondrocytes.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Huang X,Xi Y,Pan Q,Mao Z,Zhang R,Ma X,You H

Thu Nov 01 00:00:00 EDT 2018

Astragaloside exerts anti-photoaging effects in UVB-induced premature senescence of rat dermal fibroblasts through enhanced autophagy.

Archives of biochemistry and biophysics

Wen W,Chen J,Ding L,Luo X,Zheng X,Dai Q,Gu Q,Liu C,Liang M,Guo X,Liu P,Li M

Thu Nov 01 00:00:00 EDT 2018

Molecularly targeted anti-cancer drugs inhibit the invasion and metastasis of hepatocellular carcinoma by regulating the expression of MMP and TIMP gene families.

Biochemical and biophysical research communications

He XX,Shi LL,Qiu MJ,Li QT,Wang MM,Xiong ZF,Yang SL

Fri Oct 12 00:00:00 EDT 2018

Effects of growth hormone and functional appliance on mandibular growth in an adolescent rat model.

The Angle orthodontist

Wang S,Ye L,Li M,Zhan H,Ye R,Li Y,Zhao Z

Sat Sep 01 00:00:00 EDT 2018

Tumor cell density regulates matrix metalloproteinases for enhanced migration.

Oncotarget

Jayatilaka H,Umanzor FG,Shah V,Meirson T,Russo G,Starich B,Tyle P,Lee JSH,Khatau S,Gil-Henn H,Wirtz D

Fri Aug 24 00:00:00 EDT 2018

Investigation of the expressions of MMPs and TIMPs between isogeneic and allogeneic rat aortic transplantation.

Molecular medicine reports

Wei SD,Cao D,Xu XS,Bai H,Wu H,Wang MH

Tue May 01 00:00:00 EDT 2018

Chemerin suppresses hepatocellular carcinoma metastasis through CMKLR1-PTEN-Akt axis.

British journal of cancer

Li JJ,Yin HK,Guan DX,Zhao JS,Feng YX,Deng YZ,Wang X,Li N,Wang XF,Cheng SQ,Bao Y,Xie D

Tue May 01 00:00:00 EDT 2018

Myosin 1b promotes cell proliferation, migration, and invasion in cervical cancer.

Gynecologic oncology

Zhang HR,Lai SY,Huang LJ,Zhang ZF,Liu J,Zheng SR,Ding K,Bai X,Zhou JY

Sun Apr 01 00:00:00 EDT 2018

Sinapic Acid Inhibits the IL-1β-Induced Inflammation via MAPK Downregulation in Rat Chondrocytes.

Inflammation

Huang X,Pan Q,Mao Z,Zhang R,Ma X,Xi Y,You H

Thu Mar 01 00:00:00 EST 2018

Protective effects of psoralidin on IL‑1β‑induced chondrocyte apoptosis.

Molecular medicine reports

Rao Z,Wang S,Wang J

Thu Feb 01 00:00:00 EST 2018

MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN.

Journal of cellular and molecular medicine

Chen L,Li Q,Wang J,Jin S,Zheng H,Lin J,He F,Zhang H,Ma S,Mei J,Yu J

Fri Dec 01 00:00:00 EST 2017

Collagen I promotes hepatocellular carcinoma cell proliferation by regulating integrin β1/FAK signaling pathway in nonalcoholic fatty liver.

Oncotarget

Zheng X,Liu W,Xiang J,Liu P,Ke M,Wang B,Wu R,Lv Y

Fri Nov 10 00:00:00 EST 2017

Far-infrared suppresses skin photoaging in ultraviolet B-exposed fibroblasts and hairless mice.

PloS one

Chiu HW,Chen CH,Chen YJ,Hsu YH

Thu Sep 07 00:00:00 EDT 2017

Downregulation of MMP1 in MDS-derived mesenchymal stromal cells reduces the capacity to restrict MDS cell proliferation.

Scientific reports

Zhao S,Zhao Y,Guo J,Fei C,Zheng Q,Li X,Chang C

Mon Mar 06 00:00:00 EST 2017

Long Non-Coding RNA BANCR Promotes Endometrial Cancer Cell Proliferation and Invasion by Regulating MMP2 and MMP1 via ERK/MAPK Signaling Pathway.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang D,Wang D,Wang N,Long Z,Ren X

Mon Feb 13 00:00:00 EST 2017

Pigment Epithelium-Derived Factor (PEDF) mediates cartilage matrix loss in an age-dependent manner under inflammatory conditions.

BMC musculoskeletal disorders

Nakamura DS,Hollander JM,Uchimura T,Nielsen HC,Zeng L

Wed Jan 25 00:00:00 EST 2017

The trophoblast plug during early pregnancy: a deeper insight.

Histochemistry and cell biology

Weiss G,Sundl M,Glasner A,Huppertz B,Moser G

Thu Dec 01 00:00:00 EST 2016

CDK5 functions as a tumor promoter in human colorectal cancer via modulating the ERK5-AP-1 axis.

Cell death & disease

Zhuang K,Zhang J,Xiong M,Wang X,Luo X,Han L,Meng Y,Zhang Y,Liao W,Liu S

Thu Oct 13 00:00:00 EDT 2016

CDK5 functions as a tumor promoter in human colorectal cancer via modulating the ERK5-AP-1 axis.

Cell death & disease

Zhuang K,Zhang J,Xiong M,Wang X,Luo X,Han L,Meng Y,Zhang Y,Liao W,Liu S

Thu Oct 13 00:00:00 EDT 2016

Identification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma.

Cell research

Li F,Fang Z,Zhang J,Li C,Liu H,Xia J,Zhu H,Guo C,Qin Z,Li F,Han X,Wang Y,Feng Y,Wang Y,Zhang W,Wang Z,Jin Y,Sun Y,Wei W,Zeng R,Chen H,Ji H

Sat Oct 01 00:00:00 EDT 2016

Heme oxygenase-1 attenuates IL-1β induced alteration of anabolic and catabolic activities in intervertebral disc degeneration.

Scientific reports

Hu B,Shi C,Xu C,Cao P,Tian Y,Zhang Y,Deng L,Chen H,Yuan W

Mon Feb 15 00:00:00 EST 2016

Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.

PloS one

Kmiecik AM,Pula B,Suchanski J,Olbromski M,Gomulkiewicz A,Owczarek T,Kruczak A,Ambicka A,Rys J,Ugorski M,Podhorska-Okolow M,Dziegiel P

Tue Jan 26 00:00:00 EST 2016

Conditional knockout of tissue factor pathway inhibitor 2 in vascular endothelial cells accelerates atherosclerotic plaque development in mice.

Thrombosis research

Hong J,Liu R,Chen L,Wu B,Yu J,Gao W,Pan J,Luo X,Shi H

Fri Jan 01 00:00:00 EST 2016

Effects of small interfering RNA-mediated downregulation of the Krüppel-like factor 4 gene on collagen metabolism in human hepatic stellate cells.

Molecular medicine reports

Li T,Niu L,Li M,Liu Y,Xu Z,Gao X,Liu D

Tue Sep 01 00:00:00 EDT 2015

Potent effects of dioscin against liver fibrosis.

Scientific reports

Zhang X,Han X,Yin L,Xu L,Qi Y,Xu Y,Sun H,Lin Y,Liu K,Peng J

Wed Apr 08 00:00:00 EDT 2015

Protective effects of biochanin A on articular cartilage: in vitro and in vivo studies.

BMC complementary and alternative medicine

Wu DQ,Zhong HM,Ding QH,Ba L

Sat Nov 15 00:00:00 EST 2014

Celastrol, an inhibitor of heat shock protein 90β potently suppresses the expression of matrix metalloproteinases, inducible nitric oxide synthase and cyclooxygenase-2 in primary human osteoarthritic chondrocytes.

European journal of pharmacology

Ding QH,Cheng Y,Chen WP,Zhong HM,Wang XH

Wed May 15 00:00:00 EDT 2013

CD133 affects the invasive ability of HCT116 cells by regulating TIMP-2.

The American journal of pathology

Zhang M,Liu Y,Feng H,Bian X,Zhao W,Yang Z,Gu B,Li Z,Liu Y

Fri Feb 01 00:00:00 EST 2013

Anti-arthritic effects of crocin in interleukin-1β-treated articular chondrocytes and cartilage in a rabbit osteoarthritic model.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Ding Q,Zhong H,Qi Y,Cheng Y,Li W,Yan S,Wang X

Tue Jan 01 00:00:00 EST 2013

MicroRNA-34a inhibits migration and invasion of colon cancer cells via targeting to Fra-1.

Carcinogenesis

Wu J,Wu G,Lv L,Ren YF,Zhang XJ,Xue YF,Li G,Lu X,Sun Z,Tang KF

Thu Mar 01 00:00:00 EST 2012

Bioinformatics

Protein Aliases: 22 kDa interstitial collagenase; 27 kDa interstitial collagenase; Collagenase; collagenase-like A; Fibroblast collagenase; Interstitial collagenase; Interstitial collagenase A; matrix metallo protease; matrix metallopeptidase 1 (interstitial collagenase); matrix metallopeptidase 1a; matrix metallopeptidase 1a (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1; matrix metalloproteinase 1a (interstitial collagenase); Matrix metalloproteinase-1; Matrix metalloproteinase-1a; Matrix metalloproteinase1; Mcol-A; MMP; MMP-1; MMP-1a; MMPs

Gene Aliases: CLG; CLGN; Mcol-A; McolA; MMP1; Mmp1a

UniProt ID: (Human) P03956, (Mouse) Q9EPL5

Entrez Gene ID: (Human) 4312, (Rat) 300339, (Mouse) 83995

Molecular Function: metalloprotease

Function(s)

proteolysis viral process extracellular matrix disassembly collagen catabolic process positive regulation of protein oligomerization cellular protein metabolic process leukocyte migration luteinization luteolysis response to mechanical stimulus positive regulation of fibroblast migration response to inactivity positive regulation of smooth muscle cell migration cellular response to drug response to drug cellular response to lipopolysaccharide cellular response to iron(III) ion cellular response to vitamin D cellular response to alkaloid cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to peptide hormone stimulus cellular response to organic cyclic compound cellular response to fluid shear stress response to indole-3-methanol

Process(es)

endopeptidase activity metalloendopeptidase activity serine-type endopeptidase activity calcium ion binding zinc ion binding protein binding peptidase activity metallopeptidase activity hydrolase activity metal ion binding

MMP1 Polyclonal Antibody

Product Details

10371-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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