|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to internal region of human SMRTe protein|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain or lung carcinoma.
SMRT is 1495 amino acids and contains an 8 amino acid sequence that is not present in SMRTe (SMRT-extended), which contains 2514 amino acids. SMRTe contains an N-terminal sequence spanning over 1,000 amino acids that is not present in SMRT, but that shows significant similarity with N-CoR. SMRTe expression is regulated during cell cycle progression, suggesting a role for SMRTe in the regulation of cycle-specific gene expression in diverse signaling pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CTG repeat protein 26; CTG26; FLJ25011; nuclear receptor corepressor 2; silencing mediator for retinoid and thyroid hormone receptors; SMRT; SMRTE; SMRTE-tau; T3 receptor-associating factor; thyroid-, retinoic-acid-receptor-associated corepressor; TNRC14; TRAC-1; TRAC1
Gene Aliases: CTG26; N-CoR2; SMAP270; SMRT; SMRTE; SMRTE-tau; TNRC14; TRAC; TRAC-1; TRAC1
UniProt ID: (Human) Q9Y618
Entrez Gene ID: (Human) 9612
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