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NF-H Polyclonal Antibody

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NF-H Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 18934-1-AP (NF-H antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

NF-H Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed mouse brain tissue using 18934-1-AP (NF-H antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

NF-H Antibody in Western Blot (WB) — Mouse brain tissue were subjected to SDS PAGE followed by western blot with 18934-1-AP (NEFH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Details

18934-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 3 publications 3 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Immunohistochemistry (IHC)	-	View 5 publications 5 publications

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	NF-H Fusion Protein Ag13517 (1-214 aa encoded by BC014185) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.33 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody can recognize both NEFH and NEFM.

Immunogen sequence: MMSFGGADA LLGAPFAPLH GGGSLHYALA RKGGAGGTRS AAGSSSGFHS WTRTSVSSVS ASPSRFRGAG AASSTDSLDT LSNGPEGCMV AVATSRSEKE QLQALNDRFA GYIDKVRQLE AHNRSLEGEA AALRQQQAGR SAMGELYERE VREMRGAVLR LGAARGQLRL EQEHLLEDIA HVRQRLDDEA RQREEAEAAA RALARFAQEA EAARV (1-214 aa encoded by BC014185)

Target Information

Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-H has an important role in mature axons and is involved in amyotrophic lateral sclerosis (ALS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Endothelial progenitor cell-conditioned medium promotes angiogenesis and is neuroprotective after spinal cord injury.

Neural regeneration research

Wang T,Fang X,Yin ZS

Tue May 01 00:00:00 EDT 2018

ST8SIA2 promotes oligodendrocyte differentiation and the integrity of myelin and axons.

Glia

Szewczyk LM,Brozko N,Nagalski A,Röckle I,Werneburg S,Hildebrandt H,Wisniewska MB,Kuznicki J

Sun Jan 01 00:00:00 EST 2017

Methylene blue alleviates experimental autoimmune encephalomyelitis by modulating AMPK/SIRT1 signaling pathway and Th17/Treg immune response.

Journal of neuroimmunology

Wang J,Zhao C,Kong P,Bian G,Sun Z,Sun Y,Guo L,Li B

Sat Oct 15 00:00:00 EDT 2016

Treatment with NAD(+) inhibited experimental autoimmune encephalomyelitis by activating AMPK/SIRT1 signaling pathway and modulating Th1/Th17 immune responses in mice.

International immunopharmacology

Wang J,Zhao C,Kong P,Sun H,Sun Z,Bian G,Sun Y,Guo L

Sat Oct 01 00:00:00 EDT 2016

Tracking the Fragile X Mental Retardation Protein in a Highly Ordered Neuronal RiboNucleoParticles Population: A Link between Stalled Polyribosomes and RNA Granules.

PLoS genetics

El Fatimy R,Davidovic L,Tremblay S,Jaglin X,Dury A,Robert C,De Koninck P,Khandjian EW

Fri Jul 01 00:00:00 EDT 2016

Bone marrow mesenchymal stem cells repair spinal cord ischemia/reperfusion injury by promoting axonal growth and anti-autophagy.

Neural regeneration research

Yin F,Meng C,Lu R,Li L,Zhang Y,Chen H,Qin Y,Guo L

Mon Sep 15 00:00:00 EDT 2014

Transplantation of mesenchymal stem cells exerts anti-apoptotic effects in adult rats after spinal cord ischemia-reperfusion injury.

Brain research

Yin F,Guo L,Meng CY,Liu YJ,Lu RF,Li P,Zhou YB

Fri May 02 00:00:00 EDT 2014

The mechanisms of EGFR in the regulation of axon regeneration.

Cell biochemistry and function

Xu MF,Zhou H,Hu CY,Liang YQ,Hu L,Chen D

Wed Jan 01 00:00:00 EST 2014

Bioinformatics

Protein Aliases: 200 kDa neurofilament protein; NEF3; neurofilament; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NF 200 kD; NF-H; NHC

Gene Aliases: CMT2CC; KIAA0845; mKIAA0845; NEFH; NF-H; NF200; NFH

UniProt ID: (Human) P12036, (Mouse) P19246

Entrez Gene ID: (Human) 4744, (Rat) 24587, (Mouse) 380684

Function(s)

microtubule cytoskeleton organization ovarian follicle atresia axonogenesis spinal cord development hippocampus development cerebral cortex development cell projection assembly axon regeneration neurofilament bundle assembly cellular response to oxidative stress response to cocaine peripheral nervous system neuron axonogenesis axon development cellular response to estradiol stimulus regulation of organelle transport along microtubule response to sodium arsenite response to acrylamide brain development embryo development neuron projection development intermediate filament cytoskeleton organization intermediate filament bundle assembly neurofilament cytoskeleton organization

Process(es)

structural molecule activity structural constituent of cytoskeleton microtubule binding toxic substance binding kinesin binding protein kinase binding protein binding, bridging dynein binding protein heterodimerization activity nutrient reservoir activity

NF-H Polyclonal Antibody

Product Details

18934-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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