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Invitrogen
OPA1 Polyclonal Antibody
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FIGURE: 1 / 8
OPA1 Antibody (PA5-79771) in ICC/IF
Immunocytochemistry analysis of OPA1 using anti-OPA1 antibody (Product # PA5-79771). OPA1 was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-OPA1 antibody (Product # PA5-79771) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visual... View More
Product Details
PA5-79771
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human OPA1 (919-955aa EDGEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEA).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human PANC-1 whole cell, human PC-3 whole cell, human T47D whole cell, rat brain tissue, mouse brain tissue, mouse heart tissue, mouse NIH/3T3 whole cell. IHC: Mouse Liver tissue, Rat Kidney tissue, Human Lung Cancer tissue. ICC/IF: U20S cell. Flow: U20S cell.
Target Information
OPA1 is a dynamin-related GTPase that is critical for the maintenance of mitochondrial morphology and mtDNA. The most commonly associated phenotype with OPA1 mutations is heterozygous optic atrophy, a heterozygous dominant trait that causes reduced visual clarity and sometimes blindness. The disease usually begins in childhood and increases in severity throughout the life of affected individuals. Usually, this phenotype is attributed to the degeneration of optic nerve fibers. Interestingly, the same type of nerve degeneration seems to be partially causative of certain schizophrenia characteristics. OPA1 dysfunction also seems to be implicated in this case; mitochondrial networks associated with critical nerves seem to link schizophrenia and OPA1. The dysfunction is associated with issues with apoptosis and normal cellular metabolic regulation, all regulated through OPA1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: dynamin-like 120 kDa protein, mitochondrial; Dynamin-like GTPase OPA1, mitochondrial; dynamin-like guanosine triphosphatase; Large GTP-binding protein; LargeG; mitochondrial dynamin-like GTPase; mitochondrial OPA1; opa1 {ECO:0000312|EMBL:AAI11072.1}; optic atrophy 1 (autosomal dominant); optic atrophy 1 homolog; optic atrophy 1-like protein; Optic atrophy protein 1; Optic atrophy protein 1 homolog; RN protein
Gene Aliases: 1200011N24Rik; AI225888; AI847218; BERHS; KIAA0567; largeG; lilr3; MGM1; mKIAA0567; MTDPS14; NPG; NTG; OPA1
UniProt ID: (Human) O60313, (Rat) Q2TA68, (Mouse) Q8BLL3
Entrez Gene ID: (Human) 4976, (Rat) 171116, (Mouse) 74143
mitochondrial genome maintenance
mitochondrial fission
apoptotic process
mitochondrion organization
inner mitochondrial membrane organization
visual perception
mitochondrial fusion
positive regulation of mitochondrial fusion
positive regulation of neuron maturation
axonal transport of mitochondrion
negative regulation of release of cytochrome c from mitochondria
cellular senescence
positive regulation of dendrite development
negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
neural tube closure
aging
metabolic process
negative regulation of intrinsic apoptotic signaling pathway
response to stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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