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Invitrogen
Ub-K6 Polyclonal Antibody
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FIGURE: 1 / 2
Ub-K6 Antibody (PA5-120610) in WB
Western blot analysis of Ub-K6 in extracts of various cell lines. Samples were incubated with Ub-K6 Polyclonal antibody (Product # PA5-120610) using a dilution of 1:500, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Product Details
PA5-120610
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence containing polyubiquitinated protein (K6 linkage)
Conjugate
Form
Liquid
Concentration
0.64 mg/mL
Amount
64 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: RAW264.7, C6.
Target Information
Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. [Uniprot]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Polyubiquitin-B; ubiquitin C
Gene Aliases: AL033289; Rps27a; Uba52; Ubb; Ubb2; UBC
UniProt ID: (Mouse) P0CG49, (Rat) P0CG51
Entrez Gene ID: (Mouse) 22187, (Rat) 192255
male meiosis I
female meiosis I
male gonad development
female gonad development
ubiquitin homeostasis
hypothalamus gonadotrophin-releasing hormone neuron development
mitochondrion transport along microtubule
neuron projection morphogenesis
regulation of mitochondrial membrane potential
adipose tissue development
fat pad development
regulation of proteasomal protein catabolic process
seminiferous tubule development
energy homeostasis
regulation of neuron death
positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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