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Invitrogen
NSFL1C Polyclonal Antibody
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Product Details
PA5-21633
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain.
Predicted reactivity: Mouse (96%), Rat (96%), Xenopus laevis (83%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (96%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
p47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; P47; p97 cofactor p47; RP4-776F14.2; SHP1 homolog; UBX domain-containing protein 2C; unnamed protein product; XY body-associated protein XY40
Gene Aliases: dJ776F14.1; Munc-18c; NSFL1C; P47; Stxbp3a; UBX1; UBXD10; UBXN2C; XY40
UniProt ID: (Human) Q9UNZ2, (Rat) O35987, (Mouse) Q9CZ44
Entrez Gene ID: (Human) 55968, (Rat) 83809, (Mouse) 386649
autophagosome assembly
establishment of mitotic spindle orientation
ubiquitin-dependent protein catabolic process
Golgi organization
nuclear envelope reassembly
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of mitotic centrosome separation
membrane fusion
negative regulation of protein localization to centrosome
regulation of catalytic activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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