Western blot analysis of Jurkat Whole Cell Lysate using PA5-19552, PARN primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19552 staining of A431 Whole Cell Lysate at a dilution of 1 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25|
|Western Blot (WB)||1:25|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-19552 targets PARN in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-19552 immunogen is synthetic peptide conjugated to KLH derived from within residues 500 - 600 of Human PARN.
PA5-19552 detects PARN which has a predicted molecular weight of approximately 75 kDa.
The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers polyas the substrate, hence, efficiently degrades polytails of mRNAs. Exonucleolytic degradation of the polytail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.