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PCYT2 Antibody (PA1-545) in ICC/IF
Immunofluorescence analysis of PCYT2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PCYT2 Polyclonal Antibody (Product # PA1-545) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More
Product Details
PA1-545
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C(157) T K A H H S S Q E M S S E Y R E(172)
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Affinity chromatography
Storage buffer
tris with glycine, 1mg/mL BSA
Contains
0.02% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2159300
Product Specific Information
PA1-545 detects ethanolaminephosphate cytidyltransferase (ECT) protein in human, rat, mouse and non-human primate samples.
PA1-545 has successfully been used in Western blot procedures. By Western blot, this antibody detects an ~50 kDa protein representing ECT from C3H/10T1/2 cell lysates.
PA1-545 immunizing peptide corresponds to amino acid residues 157-172 from human ECT protein. This immunizing peptide (PEP-241) can be used for neutralization and control experiments.
Target Information
ECT (ethanolaminephosphate cytidyltransferase) is an important regulatory enzyme in the pathway for phosphatidylethanolamine (PE) biosynthesis. PE functions in cell signalling, membrane fusion, platelet activation, and cell cycle progression. Specialized forms of PE exist as plasmologens, natural cannabinoid anadimade, and glycosylated PE. The ECT enzyme acts in the second of three catalysis steps to produce CDP-ethanolamine from phosphoethanolamine (P-Etn). Study of ECT could help determine the role of plasmologens in disease, specifically lipid disorders such as peroxisomal diseases as well as neuronal degeneration associated with Alzheimer's disease.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CTP:phopshoethanolamine cytidylyltransferase; CTP:phosphoethanolamine cytidylyltransferase; Ethanolamine-phosphate cytidylyltransferase; Phosphorylethanolamine transferase
Gene Aliases: 1110033E03Rik; ET; PCYT2
UniProt ID: (Human) Q99447, (Rat) O88637, (Mouse) Q922E4
Entrez Gene ID: (Human) 5833, (Rat) 89841, (Mouse) 68671
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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