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Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:50-1:500 | - |
Immunocytochemistry (ICC/IF) |
1:50-1:500 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
PDPK1 Fusion Protein Ag9213 (198-556 aa encoded by BC012103) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.13 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: GKGIIHRDL KPENILLNED MHIQITDFGT AKVLSPESKQ ARANSFVGTA QYVSPELLTE KSACKSSDLW ALGCIIYQLV AGLPPFRAGN EYLIFQKIIK LEYDFPEKFF PKARDLVEKL LVLDATKRLG CEEMEGYGPL KAHPFFESVT WENLHQQTPP KLTAYLPAMS EDDEDCYGNY DNLLSQFGCM QVSSSSSSHS LSASDTGLPQ RSGSNIEQYI HDLDSNSFEL DLQFSEDEKR LLLEKQAGGN PWHQFVENNL ILKMGPVDKR KGLFARRRQL LLTEGPHLYY VDPVNKVLKG EIPWSQELRP EAKNFKTFFV HTPNRTYYLM DPSGNAHKWC RKIQEVWRQR YQSHPDAAVQ (198-556 aa encoded by BC012103)
PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) phosphorylates AGC kinases. PDPK1 activates conventional PKC and PKC zeta through phosphorylation of critical threonine residues in the activation loop. PDPK1 also phosphorylates Protein Kinase B (PKB) at threonine 308 in the presence of phosphatidylinositol-3,4,5-trisphosphate. Active Akt inactivates Glycogen Synthase Kinase 3 (GSK3), eventually leading to the dephosphorylation and activation of glycogen synthase, and the stimulation of glycogen synthesis. Because of the role that PDPK1 plays in insulin-induced glycogen synthesis and PKC activation, it is a potentially important target for metabolic drug research.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; HGNC:8809; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; OTTHUMP00000174525; OTTHUMP00000205076; PkB kinase like gene 1; PkB-like 1
Gene Aliases: PDK1; PDPK1; PDPK2; PDPK2P; PRO0461
UniProt ID: (Human) O15530
Entrez Gene ID: (Human) 5170
Molecular Function:
non-receptor serine/threonine protein kinase
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