|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal resiues of human PHD3.|
|Storage buffer||tris citrate/tris phosphate, pH 7-8|
|Contains||0.01% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-20196 detects PHD3 in human and mouse samples.
PA1-20196 has been successfully used in Western blot procedures.
The PA1-20196 immunogen is a synthetic peptide corresponding to the C-terminal resiues of human PHD3.
HIF prolyl hydroxylase 3 is a prolyl hydroxylase that modifies HIF alpha. Classic prolyl hydroxylases are found in the endoplasmic reticulum and modify collagen, whereas HIF is an intracellular protein and the HPH sites do not resemble those modifying collagen. HIF is a transcriptional complex that plays a critical role in oxygen homeostasis. HPH is an essential component of the pathway through which cells sense oxygen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.