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Invitrogen
POLH Polyclonal Antibody
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FIGURE: 1 / 2
POLH Antibody (PA5-102492) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded POLH in mouse kidney tissue. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with POLH polyclonal antibody (Product # PA5-102492) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-102492
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POLH(Accession Q9Y253), corresponding to amino acid residues Q541-T591.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total POLH.
Target Information
The XPA gene encodes a zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (POL H) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA polymerase eta; DNA-directed DNA polymerase eta; FLJ16395; FLJ21978; polymerase (DNA directed), eta; polymerase (DNA directed), eta (RAD 30 related); polymerase (DNA) eta; RAD30 homolog A; Xeroderma pigmentosum variant type protein; Xeroderma pigmentosum variant type protein homolog; XP V
Gene Aliases: POLH; RAD30; RAD30A; XP-V; XPV
UniProt ID: (Human) Q9Y253, (Mouse) Q9JJN0
Entrez Gene ID: (Human) 5429, (Rat) 316235, (Mouse) 80905
DNA synthesis involved in DNA repair
DNA replication
DNA repair
regulation of DNA repair
pyrimidine dimer repair
response to UV-C
translesion synthesis
error-free translesion synthesis
cellular response to UV-C
postreplication repair
biological_process
DNA biosynthetic process
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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