Invitrogen

PR3 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (25) PR3 antibodies

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Protocols

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PR3 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of PR3 in human bone marrow and cerebral cortex tissues using a PR3 polyclonal antibody (Product # PA5-82223). Corresponding RNA-seq data are presented for the same tissues.

PR3 Antibody in Western Blot (WB) — Western blot analysis of PR3 by a PR3 polyclonal antibody (Product # PA5-82223). Analysis in control (vector only transfected HEK293T lysate) and PRTN3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400985).

PR3 Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PR3 demonstrates antibody specificity. Immunohistochemical analysis of PR3 using anti-PR3 Polyclonal Antibody (Product #PA5-82223), shows significant staining of PR3 in bone marrow and shows minimal or weak staining in cerebral cortex tissues. The relative expression levels of PR3 within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82223

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PRTN3
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789384

Product Specific Information

Immunogen sequence: PTQQHFSVAQ VFLNNYDAEN KLNDILLIQL SSPANLSASV ATVQLPQQDQ PVPHGTQCLA MGWGRVGAHD PPAQVLQELN VTVVTFFCRP HNICTFVPRR KAGICFGDSG GPLICDGI

Target Information

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed:3198760, PubMed:2033050, PubMed:28240246). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed:23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed:22266279). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AGP7; azurophil granule protein 7; C-ANCA antigen; Leukocyte proteinase 3; Myeloblastin; Neutrophil proteinase 4; NP-4; P29; PR-3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen

Gene Aliases: ACPA; AGP7; C-ANCA; CANCA; MBN; MBT; NP-4; NP4; P29; PR-3; PR3; PRTN3

UniProt ID: (Human) P24158

Entrez Gene ID: (Human) 5657

Molecular Function: serine protease