Immunofluorescent analysis of PRMT2 showing staining in the nucleus and cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a PRMT2 polyclonal antibody (Product # PA5-34690) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment contains a sequence corresponding to a region within amino acids 8 and 296 (P55345) of PRMT2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34690 targets PRMT2 in WB applications and shows reactivity with Human samples.
The PA5-34690 immunogen is recombinant protein fragment contains a sequence corresponding to a region within amino acids 8 and 296 (P55345) of PRMT2.
Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1-dependent manner). May be involved in growth regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.