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FIGURE: 1 / 9
PRP19 Antibody (PA5-88644) in WB
Western blot analysis of PRP19 in extracts of various cell lines. Samples were incubated with PRP19 Polyclonal antibody (Product # PA5-88644) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-88644
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19
Conjugate
Unconjugated
Form
liquid
Concentration
1.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2805050
Product Specific Information
Immunogen sequence: AREALATLKP QAGLIVPQAV PSSQPSVVGA GEPMDLGELV GMTPEIIQKL QDKATVLTTE RKKRGKTVPE ELVKPEELSK YRQVASHVGL HSASIPGILA LDLCPSDTNK ILTGGADKNV VVFDKSSEQI LATLKGHTKK VTSVVFHPSQ DLVFSASPDA TIRIWSVPNA SCVQVVRAHE SAVTGLSLHA TGDYLLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAFSENGYY LATAADDSSV; Positive Samples: HepG2, Jurkat, HeLa, Mouse heart, Rat brain; Cellular Location: Cytoplasm, Lipid droplet, Nucleus, cytoskeleton, nucleoplasm, spindle
Target Information
PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: hPso4; Neuronal differentiation-related gene protein; Nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; nuclear matrix protein SNEV; Pre-mRNA-processing factor 19; prp19 beta; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; psoralen 4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor
Gene Aliases: AA617263; AL024362; D19Wsu55e; hPSO4; NMP200; PRP19; PRPF19; PSO4; SNEV; UBOX4
UniProt ID: (Human) Q9UMS4, (Mouse) Q99KP6, (Rat) Q9JMJ4
Entrez Gene ID: (Human) 27339, (Mouse) 28000, (Rat) 246216
Molecular Function:
RNA processing factor
protein polyubiquitination
spliceosomal tri-snRNP complex assembly
spliceosomal complex assembly
mRNA splicing, via spliceosome
inner cell mass cell proliferation
transcription-coupled nucleotide-excision repair
double-strand break repair via nonhomologous end joining
lipid biosynthetic process
proteasomal protein catabolic process
cellular protein localization
negative regulation of neuron differentiation
positive regulation of mRNA splicing, via spliceosome
positive regulation of astrocyte differentiation
protein K63-linked ubiquitination
signal transduction involved in DNA damage checkpoint
generation of catalytic spliceosome for first transesterification step
DNA repair
mRNA processing
cellular response to DNA damage stimulus
RNA splicing
protein ubiquitination
positive regulation of neuron differentiation
protein binding
ligase activity
ubiquitin-ubiquitin ligase activity
identical protein binding
ubiquitin protein ligase activity
ubiquitin-protein transferase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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