Invitrogen

PRP19 Polyclonal Antibody

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PRP19 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human lung cancer using PRPF19 Polyclonal antibody (Product # PA5-88644) at a dilution of 1:100 (40x lens).

PRP19 Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using PRPF19 Polyclonal antibody (Product # PA5-88644) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 30s.

PRP19 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug PRPF19 Polyclonal antibody (Product # PA5-88644). Western blot was performed from the immunoprecipitate using PRPF19 Polyclonal antibody (Product # PA5-88644) at a dilition of 1:1000.

Product Details

Tested Applications

Dilution

Immunohistochemistry (IHC)

1:50-1:200

Immunoprecipitation (IP)

1:50-1:100

Western Blot (WB)

1:500-1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 127-416 of human PRPF19

Conjugate

Unconjugated

Form

liquid

Concentration

3.7 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: AREALATLKP QAGLIVPQAV PSSQPSVVGA GEPMDLGELV GMTPEIIQKL QDKATVLTTE RKKRGKTVPE ELVKPEELSK YRQVASHVGL HSASIPGILA LDLCPSDTNK ILTGGADKNV VVFDKSSEQI LATLKGHTKK VTSVVFHPSQ DLVFSASPDA TIRIWSVPNA SCVQVVRAHE SAVTGLSLHA TGDYLLSSSD DQYWAFSDIQ TGRVLTKVTD ETSGCSLTCA QFHPDGLIFG TGTMDSQIKI WDLKERTNVA NFPGHSGPIT SIAFSENGYY LATAADDSSV; Positive Samples: HepG2, Jurkat, HeLa, Mouse heart, Rat brain; Cellular Location: Cytoplasm, Lipid droplet, Nucleus, cytoskeleton, nucleoplasm, spindle

Target Information

PSO4 is the human homolog of yeast Pso4, a gene essential for cell survival and DNA repair (Beck et al., 2008 [PubMed 18263876]).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: hPso4; Neuronal differentiation-related gene protein; Nuclear matrix protein 200; nuclear matrix protein NMP200 related to splicing factor PRP19; nuclear matrix protein SNEV; Pre-mRNA-processing factor 19; prp19 beta; PRP19/PSO4 homolog; PRP19/PSO4 pre-mRNA processing factor 19 homolog; psoralen 4; RING-type E3 ubiquitin transferase PRP19; Senescence evasion factor

Gene Aliases: AA617263; AL024362; D19Wsu55e; hPSO4; NMP200; PRP19; PRPF19; PSO4; SNEV; UBOX4

UniProt ID: (Human) Q9UMS4, (Mouse) Q99KP6, (Rat) Q9JMJ4

Entrez Gene ID: (Human) 27339, (Mouse) 28000, (Rat) 246216

Molecular Function: RNA binding protein mRNA processing factor mRNA splicing factor nucleic acid binding