Proteintech

PTP1B Polyclonal Antibody

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Antibody Testing Data (16)

PTP1B Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 16

PTP1B Antibody (11334-1-AP) in ICC/IF

Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PTP1B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

PTP1B Antibody in Immunoprecipitation (IP)

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Product Details

11334-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

View 25 publications 25 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

View 4 publications 4 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PTP1B Fusion Protein Ag1878 (1-435 aa encoded by BC015660)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MEMEKEFEQ IDKSGSWAAI YQDIRHEASD FPCRVAKLPK NKNRNRYRDV SPFDHSRIKL HQEDNDYINA SLIKMEEAQR SYILTQGPLP NTCGHFWEMV WEQKSRGVVM LNRVMEKGSL KCAQYWPQKE EKEMIFEDTN LKLTLISEDI KSYYTVRQLE LENLTTQETR EILHFHYTTW PDFGVPESPA SFLNFLFKVR ESGSLSPEHG PVVVHCSAGI GRSGTFCLAD TCLLLMDKRK DPSSVDIKKV LLEMRKFRMG LIQTADQLRF SYLAVIEGAK FIMGDSSVQD QWKELSHEDL EPPPEHIPPP PRPPKRILEP HNGKCREFFP NHQWVKEETQ EDKDCPIKEE KGSPLNAAPY GIESMSQDTE VRSRVVGGSL RGAQAASPAK GEPSLPEKDE DHALSYWKPF LVNMCVATVL TAGAYLCYRF LFNSNT (1-435 aa encoded by B C015660)

Target Information

PTP1B (PTPN1) is a tyrosine-protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. It mediates dephosphorylation of EIF2AK3/PERK and in turn inactivates the protein kinase activity of EIF2AK3/PERK. PTP1B also plays a role in CKII- and p60c-src-induced signal transduction cascades. It regulates the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. PTP1B also regulates the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Curcumin inhibits proliferation of hepatocellular carcinoma cells by blocking PTPN1 and PTPN11 expression.

Oncology letters

Zhang J,Liu Y,Wang X,Wang Z,Xing E,Li J,Wang D

Sat Jul 01 00:00:00 EDT 2023

Efficacy of Young Cinnamomum zeylanicum Blume Bark on Hyperglycemia and PTPase Activity in Type 2 Diabetes.

Cureus

Mandal A,Sharma SK,Yadav SRM,Mirza AA,Thakur MS,Jachak S,Saini S,Roy P,Kant R,Patil M

Wed Feb 01 00:00:00 EST 2023

A Theranostic Small-Molecule Prodrug Conjugate for Neuroendocrine Prostate Cancer.

Pharmaceutics

Gonzalez P,Debnath S,Chen YA,Hernandez E,Jha P,Dakanali M,Hsieh JT,Sun X

Wed Feb 01 00:00:00 EST 2023

Role of Protein Tyrosine Phosphatase 1B Inhibitor in Early Brain Injury of Subarachnoid Hemorrhage in Mice.

Brain sciences

Zhang ZH,Zhou XM,Zhang X

Thu May 18 00:00:00 EDT 2023

Role of Protein Tyrosine Phosphatase 1B Inhibitor in Early Brain Injury of Subarachnoid Hemorrhage in Mice.

Brain sciences

Zhang ZH,Zhou XM,Zhang X

Thu May 18 00:00:00 EDT 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

Wed Feb 01 00:00:00 EST 2023

HERC2 promotes inflammation-driven cancer stemness and immune evasion in hepatocellular carcinoma by activating STAT3 pathway.

Journal of experimental & clinical cancer research : CR

Liu Y,Xu Q,Deng F,Zheng Z,Luo J,Wang P,Zhou J,Lu X,Zhang L,Chen Z,Zhang Q,Chen Q,Zuo D

Wed Feb 01 00:00:00 EST 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 EDT 2023

PLXDC2 enhances invadopodium formation to promote invasion and metastasis of gastric cancer cells via interacting with PTP1B.

Clinical & experimental metastasis

Wu B,Wang YX,Wang JJ,Xiang DF,Zhang MS,Yan ZX,Wang WY,Miao JY,Lan X,Liu JJ,Li ZY,Li C,Fan JY,Liu JY,Jiang L,Xu SL,Cui YH,Qian F

Mon Aug 01 00:00:00 EDT 2022

PLXDC2 enhances invadopodium formation to promote invasion and metastasis of gastric cancer cells via interacting with PTP1B.

Clinical & experimental metastasis

Wu B,Wang YX,Wang JJ,Xiang DF,Zhang MS,Yan ZX,Wang WY,Miao JY,Lan X,Liu JJ,Li ZY,Li C,Fan JY,Liu JY,Jiang L,Xu SL,Cui YH,Qian F

Mon Aug 01 00:00:00 EDT 2022

PLXDC2 enhances invadopodium formation to promote invasion and metastasis of gastric cancer cells via interacting with PTP1B.

Clinical & experimental metastasis

Wu B,Wang YX,Wang JJ,Xiang DF,Zhang MS,Yan ZX,Wang WY,Miao JY,Lan X,Liu JJ,Li ZY,Li C,Fan JY,Liu JY,Jiang L,Xu SL,Cui YH,Qian F

Mon Aug 01 00:00:00 EDT 2022

Suppression of RIP1 activity via S415 dephosphorylation ameliorates obesity-related hepatic insulin resistance.

Obesity (Silver Spring, Md.)

Wang Y,Xiong J,Yuan Y,Peng C,Wu P,Wang Y,Lu J,Yin Y,Xu J,Chen S,Liu J

Tue Mar 01 00:00:00 EST 2022

Endotoxin tolerance induced by Porphyromonas gingivalis lipopolysaccharide alters macrophage polarization.

Microbial pathogenesis

Gu JY,Fu ZB,Liu YJ,Cao XZ,Sun Y

Tue Mar 01 00:00:00 EST 2022

MiR-135a-5p suppresses trophoblast proliferative, migratory, invasive, and angiogenic activity in the context of unexplained spontaneous abortion.

Reproductive biology and endocrinology : RB&E

Lu Y,Zhang X,Li X,Deng L,Wei C,Yang D,Tan X,Pan W,Pang L

Tue May 24 00:00:00 EDT 2022

Multi-omics analyses of tumor-associated immune-infiltrating cells with the novel immune checkpoint protein tyrosine phosphatase 1B (PTP1B) in extracellular matrix of brain-lower-grade-glioma (LGG) and uveal-melanoma (UVM).

Frontiers in immunology

Bai KH,Zhu MJ,Zhang YY,Li XP,Chen SL,Wang DW,Dai YJ

Tue Jan 10 00:00:00 EST 2023

Frontiers in immunology

Bai KH,Zhu MJ,Zhang YY,Li XP,Chen SL,Wang DW,Dai YJ

Tue Jan 10 00:00:00 EST 2023

Frontiers in immunology

Bai KH,Zhu MJ,Zhang YY,Li XP,Chen SL,Wang DW,Dai YJ

Tue Jan 10 00:00:00 EST 2023

Protein tyrosine phosphatase 1B regulates fibroblasts proliferation, motility and extracellular matrix synthesis via the MAPK/ERK signalling pathway in keloid.

Experimental dermatology

Qian L,Wang Q,Wei C,Wang L,Yang Y,Deng X,Liu J,Qi F

Tue Feb 01 00:00:00 EST 2022

Protein tyrosine phosphatase 1B regulates fibroblasts proliferation, motility and extracellular matrix synthesis via the MAPK/ERK signalling pathway in keloid.

Experimental dermatology

Qian L,Wang Q,Wei C,Wang L,Yang Y,Deng X,Liu J,Qi F

Tue Feb 01 00:00:00 EST 2022

Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis.

iScience

Takaoka S,Yanagiya A,Mohamed HMA,Higa R,Abe T,Inoue KI,Takahashi A,Stoney P,Yamamoto T

Fri Oct 22 00:00:00 EDT 2021

The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Cell death & disease

Huang T,Li X,Wang F,Lu L,Hou W,Zhu M,Miao C

Mon Mar 29 00:00:00 EDT 2021

The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Cell death & disease

Huang T,Li X,Wang F,Lu L,Hou W,Zhu M,Miao C

Mon Mar 29 00:00:00 EDT 2021

The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Cell death & disease

Huang T,Li X,Wang F,Lu L,Hou W,Zhu M,Miao C

Mon Mar 29 00:00:00 EDT 2021

The CREB/KMT5A complex regulates PTP1B to modulate high glucose-induced endothelial inflammatory factor levels in diabetic nephropathy.

Cell death & disease

Huang T,Li X,Wang F,Lu L,Hou W,Zhu M,Miao C

Mon Mar 29 00:00:00 EDT 2021

Ferulic Acid Ameliorates Hepatic Inflammation and Fibrotic Liver Injury by Inhibiting PTP1B Activity and Subsequent Promoting AMPK Phosphorylation.

Frontiers in pharmacology

Wu J,Xue X,Fan G,Gu Y,Zhou F,Zheng Q,Liu R,Li Y,Ma B,Li S,Huang G,Ma L,Li X

Wed Sep 29 00:00:00 EDT 2021

Loss of CD36 impairs hepatic insulin signaling by enhancing the interaction of PTP1B with IR.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yang P,Zeng H,Tan W,Luo X,Zheng E,Zhao L,Wei L,Ruan XZ,Chen Y,Chen Y

Wed Apr 01 00:00:00 EDT 2020

Loss of CD36 impairs hepatic insulin signaling by enhancing the interaction of PTP1B with IR.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Yang P,Zeng H,Tan W,Luo X,Zheng E,Zhao L,Wei L,Ruan XZ,Chen Y,Chen Y

Wed Apr 01 00:00:00 EDT 2020

Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.

Frontiers in cell and developmental biology

Du J,Dong Z,Tan L,Tan M,Zhang F,Zhang K,Pan G,Li C,Shi S,Zhang Y,Liu Y,Cui H

Tue Jan 05 00:00:00 EST 2021

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Thoracic cancer

Chen Y,Tang J,Lu T,Liu F

Wed Jul 01 00:00:00 EDT 2020

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Thoracic cancer

Chen Y,Tang J,Lu T,Liu F

Wed Jul 01 00:00:00 EDT 2020

CAPN1 promotes malignant behavior and erlotinib resistance mediated by phosphorylation of c-Met and PIK3R2 via degrading PTPN1 in lung adenocarcinoma.

Thoracic cancer

Chen Y,Tang J,Lu T,Liu F

Wed Jul 01 00:00:00 EDT 2020

Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.

Journal of cellular physiology

Yao QP,Liu Z,Yao AH,Liu JT,Jiang J,Chen Y,Li SS,Han Y,Jiang ZL,Qi YX

Thu Oct 01 00:00:00 EDT 2020

Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.

Aging

Shi C,Yang J,Hu L,Liao B,Qiao L,Shen W,Xie F,Zhu G

Mon Aug 03 00:00:00 EDT 2020

Therapeutic Role of Protein Tyrosine Phosphatase 1B in Parkinson's Disease via Antineuroinflammation and Neuroprotection In Vitro and In Vivo.

Parkinson's disease

Feng CW,Chen NF,Chan TF,Chen WF

Tue Jan 19 00:00:00 EST 2021

PTP1B promotes macrophage activation by regulating the NF-κB pathway in alcoholic liver injury.

Toxicology letters

Yang L,Sun YY,Liu YR,Yin NN,Bu FT,Yu HX,Du XS,Li J,Huang C

Sat Feb 01 00:00:00 EST 2020

Highly Selective Protein Tyrosine Phosphatase Inhibitor, 2,2',3,3'-Tetrabromo-4,4',5,5'-tetrahydroxydiphenylmethane, Ameliorates Type 2 Diabetes Mellitus in BKS db Mice.

Molecular pharmaceutics

Li C,Luo J,Guo S,Jia X,Guo C,Li X,Xu Q,Shi D

Mon May 06 00:00:00 EDT 2019

Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.

Cells

Inoue T,Yamakage H,Tanaka M,Kusakabe T,Shimatsu A,Satoh-Asahara N

Fri May 31 00:00:00 EDT 2019

HDAC6 interacts with PTPN1 to enhance melanoma cells progression.

Biochemical and biophysical research communications