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Invitrogen

Phospho-A-Raf/B-Raf/C-Raf (Ser471, Ser579, Ser432) Polyclonal Antibody

View all (2) A-Raf/B-Raf/C-Raf antibodies

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Product Details

PA5-143757

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding Ser-471 in human C-Raf. This sequence has high homology with similar regions in rat and mouse C-Raf, and has high homology to the conserved sites in B-Raf (Ser-579) and A-Raf (Ser-432).

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA, 50% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2942985

Product Specific Information

This antibody was cross-adsorbed to phospho-C-Raf (Ser-338) peptide before affinity purification using phospho-C-Raf (Ser-471) peptide. The purified antibody detects a band at 74 kDa* corresponding to C-Raf in western blots of human Jurkat cells treated with Calyculin A, but is not observed after lambda phosphatase treatment.

Target Information

The Ras-Raf-MAP kinase signaling pathway is involved in control of cell proliferation and differentiation. The Raf kinase family includes A-Raf, B-Raf, and C-Raf. Each family member has three highly conserved regions (CR1-3). The N-terminal CR1 contains the Ras-GTP-binding domain. The CR2 contains a negative regulatory serine residue (C-Raf (Ser259)/B-Raf(Ser365)) that may bind 14-3-3 proteins. The CR3 is the catalytic domain that contains phosphorylation sites for Raf-regulating enzymes within two segments, the N-region and the activation segment. Activation of C-Raf involves phosphorylation at many sites including Ser-338, Tyr-341, and multiple catalytic domain sites. In B-Raf, multiple phosphorylation sites have been identified, but their specific roles are uncertain. Phosphorylation of Ser-446 may prime B-Raf for activation, and Ser-446 and/or Ser-447 phosphorylation may be critical for B-Raf biological activity during PC12 differentiation. Ser-579 is required for growth factor activation and kinase activity. Thus, multiple sites of phosphorylation within Rafs may be important for regulation of their activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRAF1; C-RAF; CRaf; Craf1 transforming; murine leukemia viral (v-raf-1) oncogene homolog 1 (3611-MSV); murine sarcoma 3611 oncogene 1; p94; Proto-oncogene B-Raf; Proto-oncogene c-RAF; RAF proto-oncogene serine/threonine-protein kinase; Raf-1; Raf1; RAFB1; sarcoma 3611 oncogene; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1; v-raf-1 leukemia viral oncogene 1; v-raf-1 murine leukemia viral oncogene homolog 1

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Gene Aliases: 6430402F14Rik; 9930012E13Rik; AA120551; AA387315; AA473386; AA990557; B-raf; BB129353; BRAF; Braf-2; BRAF1; Braf2; c-Raf; C230098H17; C87398; Craf; Craf1; D6Ertd631e; D830050J10Rik; RAF; Raf-1; RAF1; RAFB1; v-Raf

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UniProt ID: (Mouse) P28028, (Mouse) Q99N57, (Rat) P11345

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Entrez Gene ID: (Rat) 114486, (Mouse) 109880, (Mouse) 110157, (Rat) 24703

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Function(s)
protein kinase activity protein serine/threonine kinase activity MAP kinase kinase kinase activity calcium ion binding protein binding ATP binding small GTPase binding mitogen-activated protein kinase kinase binding identical protein binding protein heterodimerization activity nucleotide binding receptor signaling protein activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups metal ion binding Ras GTPase binding non-receptor serine/threonine protein kinase
Process(es)
MAPK cascade activation of MAPKK activity myeloid progenitor cell differentiation protein phosphorylation visual learning positive regulation of gene expression negative regulation of fibroblast migration cell differentiation positive regulation of peptidyl-serine phosphorylation somatic stem cell population maintenance cellular response to drug regulation of cell proliferation negative regulation of apoptotic process CD4-positive, alpha-beta T cell differentiation positive T cell selection response to peptide hormone negative regulation of neuron apoptotic process alpha-beta T cell differentiation regulation of axon regeneration positive regulation of axon regeneration positive regulation of axonogenesis protein heterooligomerization positive regulation of stress fiber assembly response to cAMP long-term synaptic potentiation head morphogenesis positive regulation of ERK1 and ERK2 cascade cellular response to calcium ion positive regulation of substrate adhesion-dependent cell spreading negative regulation of synaptic vesicle exocytosis negative regulation of endothelial cell apoptotic process signal transduction glucose transport phosphorylation intracellular signal transduction establishment of protein localization to membrane response to hypoxia cellular glucose homeostasis positive regulation of protein phosphorylation negative regulation of cell proliferation negative regulation of protein complex assembly insulin secretion involved in cellular response to glucose stimulus response to muscle stretch intermediate filament cytoskeleton organization positive regulation of transcription from RNA polymerase II promoter neurotrophin TRK receptor signaling pathway death-inducing signaling complex assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors heart development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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