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Invitrogen
Phospho-A-Raf (Thr214) Polyclonal Antibody
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FIGURE: 1 / 1
Phospho-A-Raf (Thr214) Antibody (PA5-105484) in WB
Western blot analysis of Phospho-A-Raf (Thr214) in extracts from various samples. Samples were incubated with Phospho-A-Raf (Thr214) polyclonal antibody (Product # PA5-105484). Lane 1: Hela cells (heat-shock treatment), blocked with antigen-specific peptides; Lane 2: Hela cells (heat-shock treatment); Lane 3: Raw264.7 cells (serum starvation treatment).
Product Details
PA5-105484
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ARAF(Accession P10398), corresponding to amino acid residues around phosphorylated Ser214.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of A-RAF only when phosphorylated at Ser214.
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain. A-Raf, a second member of the Raf gene family of serine/ threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest level in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A-Raf proto-oncogene serine/threonine-protein kinase; A-raf-1; Oncogene ARAF1; Proto-oncogene A-Raf; Proto-oncogene A-Raf-1; Proto-oncogene Pks; raf a; Raf related protein; raf-related oncogene; Ras-binding protein; Ras-binding protein DA-Raf; RP1-230G1.1; Serine/threonine-protein kinase A-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; v-raf murine sarcoma 3611 viral oncogene-like protein; v-raf oncogene homolog 1 (murine sarcoma 3611 virus)
Gene Aliases: 1200013E08Rik; A-RAF; ARAF; ARAF1; AW495444; PKS; PKS2; RAFA1
UniProt ID: (Human) P10398, (Rat) P14056, (Mouse) P04627
Entrez Gene ID: (Human) 369, (Rat) 64363, (Mouse) 11836
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein activity
protein binding
ATP binding
metal ion binding
MAP kinase kinase kinase activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
MAPK cascade
cellular protein modification process
regulation of TOR signaling
regulation of proteasomal ubiquitin-dependent protein catabolic process
positive regulation of peptidyl-serine phosphorylation
negative regulation of apoptotic process
activation of MAPKK activity
protein phosphorylation
intracellular signal transduction
signal transduction
phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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