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Invitrogen
Phospho-ROCK2 (Ser1366) Polyclonal Antibody
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FIGURE: 1 / 10
Phospho-ROCK2 (Ser1366) Antibody (PA5-34895) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Phospho-ROCK2-(Ser1366) was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Phospho-ROCK2 (Ser1366) Polyclonal Antibody (Product # PA5-34895) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-34895
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.46 mg/mL
Amount
146 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, 293T, 293T transfected with wild type (WT) plasmids.
Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha; unnamed protein product
Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha
UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336
Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878
nucleotide binding
protease binding
RNA binding
protein kinase activity
protein serine/threonine kinase activity
structural molecule activity
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
small GTPase binding
metal ion binding
tau protein binding
tau-protein kinase activity
endopeptidase activator activity
Rho-dependent protein serine/threonine kinase activity
protein serine kinase activity
Rho GTPase binding
poly(A) RNA binding
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
mitotic cytokinesis
epithelial to mesenchymal transition
response to ischemia
aortic valve morphogenesis
protein phosphorylation
smooth muscle contraction
signal transduction
Rho protein signal transduction
positive regulation of cardiac muscle hypertrophy
positive regulation of gene expression
negative regulation of gene expression
positive regulation of centrosome duplication
negative regulation of angiogenesis
actin cytoskeleton organization
regulation of cell adhesion
positive regulation of cell migration
cortical actin cytoskeleton organization
actomyosin structure organization
regulation of neurological system process
positive regulation of connective tissue growth factor production
regulation of actin cytoskeleton organization
regulation of circadian rhythm
stress fiber assembly
positive regulation of MAPK cascade
modulation by host of viral process
negative regulation of nitric oxide biosynthetic process
regulation of keratinocyte differentiation
positive regulation of phosphate metabolic process
rhythmic process
embryonic morphogenesis
centrosome duplication
regulation of stress fiber assembly
positive regulation of stress fiber assembly
regulation of focal adhesion assembly
mRNA destabilization
negative regulation of biomineral tissue development
response to transforming growth factor beta
protein localization to plasma membrane
positive regulation of fibroblast growth factor production
regulation of blood vessel diameter
regulation of angiotensin-activated signaling pathway
negative regulation of protein localization to lysosome
regulation of cellular response to hypoxia
regulation of cell junction assembly
regulation of beta-amyloid formation
positive regulation of beta-amyloid formation
positive regulation of protein localization to early endosome
positive regulation of amyloid precursor protein catabolic process
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
cellular response to acetylcholine
positive regulation of connective tissue replacement
response to angiotensin
regulation of establishment of cell polarity
regulation of cell motility
neural tube closure
extrinsic apoptotic signaling pathway via death domain receptors
chromosome condensation
dendrite morphogenesis
phosphorylation
intracellular signal transduction
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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