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Invitrogen
Phospho-ROCK2 (Ser1366) Polyclonal Antibody
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FIGURE: 1 / 10
Phospho-ROCK2 (Ser1366) Antibody (PA5-34895) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of Phospho-ROCK2-(Ser1366) was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Phospho-ROCK2 (Ser1366) Polyclonal Antibody (Product # PA5-34895) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-34895
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.06 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, 293T, 293T transfected with wild type (WT) plasmids.
Predicted reactivity: Mouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ROCK2, the predominant ROCK isoform in skeletal muscle, is an important regulator downstream of Rho GTPases. ROCK2 regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: p150 ROK-alpha; p164 ROCK-2; Rho kinase 2; Rho-associated coiled-coil forming kinage 2; Rho-associated coiled-coil forming kinase 2; Rho-associated protein kinase 2; Rho-associated, coiled-coil-containing protein kinase 2; Rho-associated, coiled-coil-containing protein kinase II; RhoA - binding serine/threosine kinase alpha (ROK - alpha); RhoA-binding kinase 2; ROCK II; ROCK-II; rockii; ROKalpha
Gene Aliases: B230113H15Rik; KIAA0619; mKIAA0619; Rho-kinase; ROCK-II; ROCK2; Rock2m; ROK; ROKalpha
UniProt ID: (Human) O75116, (Rat) Q62868, (Mouse) P70336
Entrez Gene ID: (Human) 9475, (Rat) 25537, (Mouse) 19878
protein serine/threonine kinase activity
structural molecule activity
protein binding
ATP binding
Rho GTPase binding
poly(A) RNA binding
metal ion binding
Rho-dependent protein serine/threonine kinase activity
kinase activity
nucleotide binding
protein kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
cytokinesis
positive regulation of protein phosphorylation
protein phosphorylation
smooth muscle contraction
I-kappaB kinase/NF-kappaB signaling
Rho protein signal transduction
positive regulation of endothelial cell migration
positive regulation of gene expression
positive regulation of centrosome duplication
negative regulation of angiogenesis
regulation of cell adhesion
cortical actin cytoskeleton organization
regulation of actin cytoskeleton organization
negative regulation of myosin-light-chain-phosphatase activity
regulation of circadian rhythm
regulation of keratinocyte differentiation
vascular endothelial growth factor receptor signaling pathway
ephrin receptor signaling pathway
rhythmic process
centrosome duplication
regulation of stress fiber assembly
regulation of focal adhesion assembly
cellular response to testosterone stimulus
establishment of protein localization to plasma membrane
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
regulation of establishment of cell polarity
regulation of cell motility
neural tube closure
extrinsic apoptotic signaling pathway via death domain receptors
actin cytoskeleton organization
chromosome condensation
dendrite morphogenesis
phosphorylation
intracellular signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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