PA1-773 detects recombinant rat and mouse rab 3D. This antibody does not detect rab 3A, 3B, or 3C.
PA1-773 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~29 kDa protein representing recombinant mouse rab 3D.
PA1-773 immunogen is a synthetic peptide corresponding to residues M(1) A S A S E P P A S P R D A A D Q N(18) of rat RAB3D. This sequence is completely conserved in mouse. This immunizing peptide (Cat. # PEP-144) is available for use in neutralization and control experiments.
Rab proteins are low-molecular-weight GTP-binding proteins that form the largest branch of the Ras superfamily of GTPases. Located on the cytoplasmic face of organelles and vesicles, rab proteins are involved in intracellular membrane fusion reactions. Three membrane proteins, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin, form the core of a ubiquitous membrane fusion machine that interacts with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and a-SNAP. Rab proteins, in coordination with the core fusion machinery and Munc-18, help to mediate vesicle docking and fusion. There exist over 40 rab proteins that have been described in mammals. Rab 3D has been shown to be present, in part, in the tongue, pancreas, and liver.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GTP-binding protein Rab-3D; Ras-Associated Protein 3D; Ras-related protein Rab-3D
Gene Aliases: C130057E11Rik; Rab16; Rab3d