MA1-810A detects retinoic acid receptor (RAR) alpha from human, rat and mouse tissues and overexpressed cells. This antibody does not detect any of the RAR beta or gamma forms.
MA1-810A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a single ~45 kDa protein representing RAR alpha in transfected COS cells. Due to the extremely low levels of RAR expressed in native tissues, it is recommended that sensitive detection systems, such as enhanced chemiluminescence, be used.
The apparent molecular weight of RAR alpha from transfected COS cells is ~45 kDa. In rat tissues, such as skin and testis, doublets in the ~50 kDa area are seen. RAR alpha has been identified with this antibody in human dermal and neonatal fibroblast cell lines, as well as mouse F9 and human SH-SY5Y cells; however, RAR alpha from human leukocytes and HUT102 cells were not identified by MA1-810 suggesting that there may be structural variation of RAR alpha between some cell types.
The MA1-810A immunogen is a synthetic peptide corresponding to residues C(444) S P S L S P S S H R S S P A T Q S P(462) of mouse RAR alpha. This peptide (Cat. # PEP-004) is available for use in neutralization and control experiments.
Reconstitute with PBS.
RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Nuclear receptor subfamily 1 group B member 1; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RAR Alpha; RAR alpha 1; RAR-alpha; retinoic acid; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; retinoic acid receptor; Retinoic acid receptor alpha; retinoic acid receptor, alpha polypeptide; RRA
Gene Aliases: NR1B1; RAR; RARA; RARalpha1
Molecular Function: C4 zinc finger nuclear receptor