Invitrogen

RARA Monoclonal Antibody (Ralpha10)

3 Published Figures
4 References
View all (34) RARA antibodies

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RARA Monoclonal Antibody (Ralpha10)

Product Details

MA1-810A

Applications
Tested Dilution
Publications

Western Blot (WB)

1:100-1:1,000
View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:10-1:200
View 1 publication 1 publication

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

Ralpha10

Immunogen

Synthetic peptide corresponding to residues C(444) S P S L S P S S H R S S P A T Q S P(462) of mouse RAR alpha.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11157462

Product Specific Information

MA1-810A detects retinoic acid receptor (RAR) alpha from human, rat and mouse tissues and overexpressed cells. This antibody does not detect any of the RAR beta or gamma forms.



MA1-810A has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a single ~45 kDa protein representing RAR alpha in transfected COS cells. Due to the extremely low levels of RAR expressed in native tissues, it is recommended that sensitive detection systems, such as enhanced chemiluminescence, be used.



The apparent molecular weight of RAR alpha from transfected COS cells is ~45 kDa. In rat tissues, such as skin and testis, doublets in the ~50 kDa area are seen. RAR alpha has been identified with this antibody in human dermal and neonatal fibroblast cell lines, as well as mouse F9 and human SH-SY5Y cells; however, RAR alpha from human leukocytes and HUT102 cells were not identified by MA1-810 suggesting that there may be structural variation of RAR alpha between some cell types.



The MA1-810A immunogen is a synthetic peptide corresponding to residues C(444) S P S L S P S S H R S S P A T Q S P(462) of mouse RAR alpha. This peptide (Cat. # PEP-004) is available for use in neutralization and control experiments.



Reconstitute with PBS.

Target Information

RARA is a receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, the RXR-RAR heterodimers associate with a multiprotein complex containing transcription corepressors that induce histone acetylation, chromatin condensation and transcriptional suppression. On ligand binding, the corepressors dissociate from the receptors and associate with the coactivators leading to transcriptional activation. RARA plays an essential role in the regulation of retinoic acid-induced germ cell development during spermatogenesis. Has a role in the survival of early spermatocytes at the beginning prophase of meiosis. In Sertoli cells, may promote the survival and development of early meiotic prophase spermatocytes. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. Regulates expression of target genes in a ligand-dependent manner by recruiting chromatin complexes containing KMT2E/MLL5. Mediates retinoic acid-induced granulopoiesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Nuclear receptor subfamily 1 group B member 1; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RAR Alpha; RAR alpha 1; RAR-alpha; retinoic acid; retinoic acid nuclear receptor alpha variant 1; retinoic acid nuclear receptor alpha variant 2; retinoic acid receptor; Retinoic acid receptor alpha; retinoic acid receptor, alpha polypeptide; RRA

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Gene Aliases: NR1B1; RAR; RARA; RARalpha1

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UniProt ID: (Human) P10276, (Mouse) P11416

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Entrez Gene ID: (Human) 5914, (Rat) 24705, (Mouse) 19401

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Function(s)
translation repressor activity, nucleic acid binding RNA polymerase II regulatory region sequence-specific DNA binding retinoic acid binding transcription factor activity, sequence-specific DNA binding steroid hormone receptor activity retinoic acid receptor activity transcription coactivator activity transcription corepressor activity receptor binding protein binding transcription factor binding drug binding zinc ion binding enzyme binding protein domain specific binding chromatin DNA binding histone deacetylase binding protein kinase B binding retinoic acid-responsive element binding protein heterodimerization activity mRNA 5'-UTR binding protein kinase A binding alpha-actinin binding RNA polymerase II regulatory region DNA binding DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding phosphatidylinositol 3-kinase regulator activity sequence-specific DNA binding transcription regulatory region DNA binding regulatory region DNA binding metal ion binding C4 zinc finger nuclear receptor
Process(es)
negative regulation of transcription from RNA polymerase II promoter ureteric bud development neural tube closure liver development glandular epithelial cell development outflow tract septum morphogenesis growth plate cartilage development transcription initiation from RNA polymerase II promoter protein phosphorylation signal transduction germ cell development spermatogenesis female pregnancy positive regulation of cell proliferation negative regulation of cell proliferation hippocampus development prostate gland development negative regulation of granulocyte differentiation embryonic camera-type eye development regulation of myelination response to estradiol response to retinoic acid negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production response to vitamin A response to cytokine multicellular organism growth negative regulation of apoptotic process apoptotic cell clearance steroid hormone mediated signaling pathway response to ethanol positive regulation of T-helper 2 cell differentiation positive regulation of neuron differentiation positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter negative regulation of translational initiation regulation of synaptic plasticity retinoic acid receptor signaling pathway positive regulation of binding ventricular cardiac muscle cell differentiation Sertoli cell fate commitment limb development face development trachea cartilage development chondroblast differentiation negative regulation of cartilage development cellular response to lipopolysaccharide cellular response to retinoic acid cellular response to estrogen stimulus transcription, DNA-templated regulation of transcription, DNA-templated transcription from RNA polymerase II promoter male gonad development positive regulation of gene expression negative regulation of gene expression positive regulation of phosphatidylinositol 3-kinase signaling negative regulation of translation intracellular estrogen receptor signaling pathway regulation of granulocyte differentiation regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of cell differentiation positive regulation of protein kinase B signaling bone development positive regulation of ERK1 and ERK2 cascade
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