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Invitrogen
RFC1 Polyclonal Antibody
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FIGURE: 1 / 3
RFC1 Antibody (PA5-102446) in IHC (P)
Immunohistochemistry analysis of RFC1 in mouse heart tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with RFC1 polyclonal antibody (Product # PA5-102446) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-102446
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RFC1(Accession P35251), corresponding to amino acid residues S259-E309.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RFC1.
Target Information
Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism, including DNA replication, DNA repair and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3 and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. RFC2, the third-largest subunit of the RFC complex, exhibits ATP binding which makes it important for both DNA replication and checkpoint function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: A1 140 kDa subunit; A1-P145; Activator 1 140 kDa subunit; Activator 1 large subunit; Activator 1 subunit 1; Differentiation-specific element-binding protein; DNA-binding protein PO-GA; ISRE-binding protein; MHC binding factor, beta; replication factor C (activator 1) 1, 145kDa; replication factor C 1; Replication factor C 140 kDa subunit; Replication factor C large subunit; Replication factor C subunit 1; replication factor C, 140 kDa; replication factor C1; RF-C 140 kDa subunit
Gene Aliases: 140kDa; A1; Alp145; Ibf-1; MHCBFB; PO-GA; RECC1; RFC; RFC1; RFC140
UniProt ID: (Human) P35251, (Mouse) P35601
Entrez Gene ID: (Human) 5981, (Rat) 89809, (Mouse) 19687
telomere maintenance via recombination
DNA-dependent DNA replication
transcription-coupled nucleotide-excision repair
nucleotide-excision repair, DNA incision, 5'-to lesion
nucleotide-excision repair, DNA gap filling
transcription, DNA-templated
regulation of transcription, DNA-templated
telomere maintenance via telomerase
translesion synthesis
nucleotide-excision repair, DNA incision
error-prone translesion synthesis
DNA damage response, detection of DNA damage
positive regulation of catalytic activity
error-free translesion synthesis
negative regulation of transcription from RNA polymerase II promoter
DNA replication
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
DNA repair
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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