Immunofluorescent analysis of SEC14L2 showing staining in the cytoplasm and nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a SEC14L2 polyclonal antibody (Product # PA5-30473) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 37 and 250 of Human SEC14L2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30473 targets SEC14L2 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-30473 immunogen is recombinant fragment corresponding to a region within amino acids 37 and 250 of Human SEC14L2.
This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
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