Immunohistochemical analysis of SFRP1 in formalin-fixed human dermal hypertrophic scar tissue using a SFRP1 polyclonal antibody (Product # PA1-29540) at a dilution of 1:800 for 20 min. Heat-mediated antigen retrieval was performed prior to blocking, and secondary detection was performed using an HRP Goat-anti-Rabbit IgG antibody.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide IGPYQSGRFYTKC, corresponding to amino acids 42 - 53 of Human SFRP1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:200-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29540 detects SFRP1 from human samples.
PA1-29540 has been successfully used in Western blot applications.
The PA1-29540 immunogen is: Synthetic peptide: IGPYQSGRFYTKC, corresponding to amino acids 42 - 53 of Human SFRP1.
This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.