|Tested species reactivity||Bovine, Dog, Human, Mouse, Non-human primate, Pig, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A portion of residues 100-150 of human SMAD3.|
|Storage buffer||PBS with 0.2% gelatin|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||0.5-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to react with zebrafish, xenopus and chicken based on 90% sequence homology.
Suggested positive control: human liver protein, liver lysate.
Principal Names: Mothers against Decapentaplegic homolog 3 (Drosophila); SMAD3; hMAD-3; hSMAD3; HSPC193; JV15-2; MADH3; MGC60396; Official Gene Symbol: SMAD3 Gen Bank Accession Number: NP_005893 Gene ID: 4088 (Human) Gene Map Locus: 15q22.33 (Human) SMAD3, a member of SMAD family of proteins, is involved in TGF-beta signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-beta and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.